首页|Expression of HER2 and bradykinin B1 receptors in precursor lesions of gallbladder carcinoma
Expression of HER2 and bradykinin B1 receptors in precursor lesions of gallbladder carcinoma
扫码查看
点击上方二维码区域，可以放大扫码查看
原文链接
NETL
NSTL
万方数据
维普
AIM:TO determine the expression of HER2 and bradykinin B1 receptors (B1R) in the two pathogenic models of gallbladder cancer:the metaplasia-dysplasia-carcinoma and the adenoma-carcinoma pathways.METHODS:Receptor proteins were visualized by immunohistochemistry on 5-μm sections of paraffin-embedded tissue.Expression of both receptors was studied in biopsy samples from 92 patients (6 males and 86 females; age ranging from 28 to 86 years,mean 56 years).High HER2 expression in specimens was additionally investigated by fluorescence in situ hybridization.Cell proliferation in each sample was assessed by using the Ki-67 proliferation marker.RESULTS:HER2 receptor protein was absent in adenomas and in normal gallbladder epithelium.On the contrary,there was intense staining for HER2 on the basolateral membrane of epithelial cells of intestinal metaplasia (22/24; 91.7％) and carcinoma in situ (9/10;90％),the lesions that displayed a significantly high proliferation index.Protein up-regulation of HER2 in the epithelium with metaplasia or carcinoma in situ was not accompanied by HER2 gene amplification.A similar result was observed in invasive carcinomas (0/12).The B1R distribution pattern mirrored that of HER2 except that B1R was additionally observed in the adenomas.The B1R appeared either as cytoplasmic dots or labelingon the apical cell membrane of the cells composing the epithelia with intestinal metaplasia (24/24; 100％) and carcinoma in situ (10/10; 100％) and in the epithelial cells of adenomas.In contrast,both HER2 (4/12; 33％)and B1R (1/12; 8.3％) showed a low expression in invasive gallbladder carcinomas.CONCLUSION:The up-regulation of HER2 and B1R in precursor lesions of gallbladder carcinoma suggests cross-talk between these two receptors that may be of importance in the modulation of cell proliferation in gallbladder carcinogenesis.
Bradykinin B1 receptorHER2Gallbladder adenomaGallbladder dysplasiaIntestinal metaplasiaGallbladder cancer
Cesar Toledo、Carola E Matus、Ximena Barraza、Pamela Arroyo、Pamela Ehrenfeld、Carlos D Figueroa、Kanti D Bhoola、Maeva del Pozo、Maria T Poblete
展开 >
Laboratorio de Patologia Celular, Instituto de Anatomia, Histologia y Patologia,Universidad Austral de Chile, Valdivia Box 567, Chile
Servicio de Anatomia Patologica, Hospital Base, Valdivia 5090145, Chile
Lung Institute of Western Australia, Centre for Asthma and Allergy Research Institute, University of Western Australia, Perth 6006, Australia
National Fund for Development of Science and Technology,FONDECYT,Chile
NETL
NSTL
万方数据
维普
