首页|Proteomic analysis of gastric cancer and immunoblot validation of potential biomarkers
Proteomic analysis of gastric cancer and immunoblot validation of potential biomarkers
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NETL
NSTL
万方数据
维普
AIM:To search for and validate differentially expressed proteins in patients with gastric adenocarcinoma.METHODS:We used two-dimensional gel electrophoresis and mass spectrometry to search for differentially expressed proteins in patients with gastric adenocarcinoma.A set of proteins was validated with immunoblotting.RESULTS:We identified 30 different proteins involved in various biological processes:metabolism,development,death,response to stress,cell cycle,cell communication,transport,and cell motility.Eight proteinswere chosen for further validation by immunoblotting.Our results show that gastrokine-1,39S ribosomal protein L12 (mitochondrial precursor),plasma cell-induced resident endoplasmic reticulum protein,and glutathione S-transferase mu 3 were significantly underexpressed in gastric adenocarcinoma relative to adjacent non-tumor tissue samples.On the other hand,septin-2,ubiquitin-conjugating enzyme E2 N,and transaldolase were significantly overexpressed.Translationally controlled tumor protein was shown to be differentially expressed only in patients with cancer of the gastric cardia/esophageal border.CONCLUSION:This work presents a set of possible diagnostic biomarkers,validated for the first time.It might contribute to the efforts of understanding gastric cancer carcinogenesis.
Two-dimensional gel electrophoresisMass spectrometryGastric adenocarcinomaProteomeBiomarkers
Medical Centre for Molecular Biology, Institute of Biochemistry, Faculty of Medicine, University of Ljubljana, Ljubljana 1000, Slovenia
International Centre for Genetic Engineering and Biotechnology Trieste, AREA Science Park, Trieste 34149, Italy
Department of Obstetrics and Gynecology, Department of Gynecological Pathology and Cytology,University Clinical Hospital Ljubljana, Ljubljana 1000, Slovenia
NETL
NSTL
万方数据
维普
