首页|Increased presence of effector lymphocytes during Helicobacter hepaticus-induced colitis

Increased presence of effector lymphocytes during Helicobacter hepaticus-induced colitis

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AIM:To identify and characterize drosophila mothers against decapentaplegic (SMAD)3-dependent changes in immune cell populations following infection with HeliCobacter hepaticus (H.hepaticus).METHODS:SMAD3-/-(n =19) and colitis-resistant SMAD3+/-(n =24) mice (8-10 wk of age) were infected with H.hepaticus and changes in immune cell populations [T lymphocytes,natural killer (NK) cells,T regulatory cells] were measured in the spleen and mesenteric lymph nodes (MsLNs) at 0 d,3 d,7 d and 28 d post-infection using flow cytometry.Genotypedependent changes in T lymphocytes and granzyme B+ cells were also assessed after 28 d in proximal colon tissue using immunohistochemistry.RESULTS:As previously observed,SMAD3-/-,but not SMAD3+/-mice,developed colitis,peaking at 4 wk post-infection.No significant changes in T cell subsets were observed in the spleen or in the MsLNs between genotypes at any time point.However,CD4+ and CD8+/CD62L10 cells,an effector T lymphocyte population,as well as NK cells (NKp46/DX5+) were significantly higher in the MsLNs of SMAD3-/-mice at 7 d and 28 d post-infection.In the colon,a higher number of CD3+ cells were present in SMAD3-/-compared to SMAD3+/-mice at baseline,which did not significantly change during infection.However,the number of granzyme B+ cells,a marker of cytolytic lymphocytes,significantly increased in SMAD3-/-mice 28 d post-infection compared to both SMAD3+/-mice and to baseline values.This was consistent with more severe colitis development in these animals.CONCLUSION:Data suggest that defects in SMAD3signaling increase susceptibility to H.hepaticus-induced colitis through aberrant activation and/or dysregulation of effector lymphocytes.

Transforming growth factor-βColitisDrosophila mothers against decapentaplegicColon cancerT lymphocytes

Sarah J McCaskey、Elizabeth A Rondini、Jonathan F Clinthorne、Ingeborg M Langohr、Elizabeth M Gardner、Jenifer I Fenton

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Department of Food Science and Human Nutrition, College of Osteopathic Medicine,Michigan State University, East Lansing, MI 48824, United States

Department of Food Science Human Nutrition, Michigan State University, East Lansing, MI 48824, United States

Department of Pathobiology and Diagnostic Investigation, Michigan State University, East Lansing,MI 48824, United States

2012

10.3748/wjg.v18.i13.1459

世界胃肠病学杂志(英文版)
太原消化病研治中心

世界胃肠病学杂志(英文版)

SCI
影响因子：1.001
ISSN：1007-9327
年,卷(期)：2012.18(13)
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