首页|Toxicarioside A inhibits SGC-7901 proliferation, migration and invasion via NF-κB/bFGF signaling

Toxicarioside A inhibits SGC-7901 proliferation, migration and invasion via NF-κB/bFGF signaling

扫码查看
原文链接
  • NETL
  • NSTL
  • 万方数据
AIM:To investigate the inhibitory role of toxicarioside A on the gastric cancer cell line human gastric cancer cell line (SGC-7901) and determine the underlying molecular mechanism.METHODS:After SGC-7901 cells were treated with toxicarioside A at various concentrations (0.5,1.5,4.5,9.0 μg/mL) for 24 h or 48 h,cell viability was determined by 3-(4,5-dimethyl-thiazol-2-yl)-2,5-diphenyl-2H-tetrazolium bromide assay,and the motility and invasion of tumor cells were assessed by the Transwell chamber assay.Immunofluorescence staining,reverse transcription polymerase chain reaction and Western blotting were performed to detect the expression of basic fibroblast growth factor (bFGF) and fibroblast growth factor receptor-1 (FGFR1),and nuclear factorkappa B (NF-κB) activation was examined by electrophoretic mobility shift assay.RESULTS:The results showed that toxicarioside A was capable of reducing cell viability,inhibiting cell growth,and suppressing cell migration and invasion activities in a time-and dose-dependent manner in SGC-7901cells.Further analysis revealed that not only the expression of bFGF and its high-affinity receptor FGFR1 but also the NF-κB-DNA binding activity were effectively blocked by toxicarioside A in a dose-dependent manner compared with the control group (P ＜ 0.05 or P ＜ 0.01).Interestingly,application of the NF-κB specific inhibitor,pyrrolidinedithiocarbamate (PDTC),to SGC-7901 cells significantly potentized the toxicarioside A-induced down-regulation of bFGF compared with the control group (P ＜ 0.05).CONCLUSION:These findings suggest that toxicarioside A has an anti-gastric cancer activity and this effect may be achieved partly through down-regulation of NF-κB and bFGF/FGFR1 signaling.

Anti-migrationAnti-proliferationBasic fibroblast growth factorGastric cancerNuclear factorkappa BToxicarioside A

Jun-Li Guo、Shao-Jiang Zheng、Yue-Nan Li、Wei Jie、Xin-Bao Hao、Tian-Fa Li、Li-Ping Xia

展开 >

Hainan Provincial Key Laboratory of Tropical Medicine, Cancer Research Institute of Affiliated Hospital, Hainan Medical College, Haikou 571199, Hainan Province, China

Department of Pathology, Guangdong Medical College, Zhanjiang 524023, Guangdong Province, China

Grants from the National Natural Scientific Foundation of ChinaNatural Foundation of Hainan Province of ChinaNatural Foundation of Hainan Province of China教育部新世纪优秀人才支持计划国家重点基础研究发展规划(973计划)Hainan Provincial Key Scientific Project

8106018430864811201NCET-08-06572010CB534909061009

2012

10.3748/wjg.v18.i14.1602

世界胃肠病学杂志(英文版)
太原消化病研治中心

世界胃肠病学杂志(英文版)

SCI
影响因子：1.001
ISSN：1007-9327
年,卷(期)：2012.18(14)
  • 9
  • 40