首页|Mucosa-associated bacteria in two middle-aged women diagnosed with collagenous colitis

Mucosa-associated bacteria in two middle-aged women diagnosed with collagenous colitis

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  • NSTL
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AIM:To characterize the colon microbiota in two women histologically diagnosed with collagenous colitis using a culture-independent method.METHODS:Biopsies were taken from the ascending colon and the total DNA was extracted.Universal bacterial primers were used to amplify the bacterial 16S rRNA genes.The amplicons were then cloned into competent Escherichia coli cells.The clones were sequenced and identified by comparison to known sequences.RESULTS:The clones could be divided into 44 different phylotypes.The microbiota was dominated by Firmicutes and Bacteroidetes.Seven phylotypes were found in both patients and constituted 47.5％ of the total number of clones.Of these,the most dominating were clones similar to Bacteroides cellulosilyticus,Bacteroides caccae,Bacteroides thetaiotaomicron,Bacteroides uniformis and Bacteroides dorei within Bacteroidetes.Sequences similar to Faecalibacterium prausnitzii and C/ostridium citroniae were also found in both patients.CONCLUSION:A predominance of potentially pathogenic Bacteroidesspp.,and the presence of clones showing similarity to Clostridium clostridioforme were found but the overall colon microbiota showed similarities to a healthy one.Etiologies for collagenous colitis other than an adverse bacterial flora must also be considered.

Microscopic colitisCollagenous colitisLymphocytic colitisColonic microbiota16S rRNA sequencing

Rita J Gustafsson、Bodil Ohlsson、Cecilia Benoni、Bengt Jeppsson、Crister Olsson

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Department of Clinical Sciences, Gastroenterology Section, Sk(a)ne University Hospital, Lund University, 20502 Malm(o), Sweden

Department of Surgery, Sk(a)ne University Hospital, Lund University, 20502 Malm(o), Sweden

Food Technology Engineering and Nutrition,Lund University, 22100 Lund, Sweden

2012

10.3748/wjg.v18.i14.1628

世界胃肠病学杂志(英文版)
太原消化病研治中心

世界胃肠病学杂志(英文版)

SCI
影响因子：1.001
ISSN：1007-9327
年,卷(期)：2012.18(14)
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