首页|Differential expression of Rab27A/B correlates with clinical outcome in hepatocellular carcinoma

Differential expression of Rab27A/B correlates with clinical outcome in hepatocellular carcinoma

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AIM:To investigate the association of Rab27A and Rab27B expression with clinicopathological characteristics and prognosis of hepatocellular carcinoma (HCC).METHODS:We used reverse transcription polymerase chain reaction (RT-PCR),real-time PCR,and Western blotting to detect Rab27A and Rab27B mRNA and protein expression in 5 human HCC lines and the immortalized hepatic HL-7702 cell line.We further examined 148 primary HCC samples matched with adjacent normal tissue and 80 non-HCC specimens by immunohistochemistry to evaluate the correlation of Rab27A and Rab27B expression with clinicopathological features and prognosis.RESULTS:Our data showed that Rab27A and Rab27Bwere differentially expressed in cell lines and primary HCC tumors.Rab27A mRNA and protein were detected in 67％ (4/6) of human cell lines and 80％ (4/5) of HCC cell lines,while Rab27B was found in 50％ (3/6) of human lines and 40％ (2/5) of HCC lines.Rab27A expression was higher in primary HCC (46.2％,66/143) than in matched adjacent tissue (24.3％,33/136,P ＜ 0.001),whereas immunopositivity for Rab27B was lower in primary HCC (57.4％,81/141) than in matched adjacent tissue (87.5％,119/136,P ＜ 0.001).Analysis of clinicopathological characteristics of 148 HCC specimens revealed significant correlations between Rab27A and Rab27B expression and tumor tumor-node-metastasis (TNM) classification (P =0.046 and P =0.027,respectively),and between strong Rab27A expression and tumor differentiation grade (P =0.008).Survival analyses revealed that patients with Rab27A+ or Rab27B+ tumors had significantly reduced overall survival compared with that of patients with Rab27A or Rab27B tumors (P =0.015 and P =0.005,respectively).Risk analyses revealed that Rab27B+ and TNM Ⅲ-Ⅳ were independent poor prognosis factors associated with a 3.36-and 3.37-fold higher relative risk of death,respectively.CONCLUSION:Rab27A and Rab27B expression were closely correlated with tumor progression and can be valuable prognostic indicators for HCC patients.

Rab27ARab27BHepatocellular carcinomaImmunohisto-chemistryPrognosis

Wei-Wei Dong、Quan Mou、Jian Chen、Jian-Tao Cui、Wen-Mei Li、Wen-Hua Xiao

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Department of Oncology,the First Affiliated Hospital, the General Hospital of Chinese People's Liberation Army, Beijing 100048, China

Department of surgery, 252 Hospital of Chinese People's Liberation Army, Baoding 071000, Hebei Province, China

Laboratory of Molecular Oncology, Peking University Cancer Hospital/Institute, Beijing 100142,China

2012

10.3748/wjg.v18.i15.1806

世界胃肠病学杂志(英文版)
太原消化病研治中心

世界胃肠病学杂志(英文版)

SCI
影响因子：1.001
ISSN：1007-9327
年,卷(期)：2012.18(15)
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