首页|KISS-1 inhibits the proliferation and invasion of gastric carcinoma cells
KISS-1 inhibits the proliferation and invasion of gastric carcinoma cells
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NETL
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维普
AIM:To investigate the function of the KISS-1 gene in gastric carcinoma cells and to explore its potential mechanism.METHODS:A KISS-1 eukaryotic expression vector was constructed and transfected into BGC-823 cells.Resistant clones were obtained through G418 selection.reverse transcription-polymerase chain reaction and western blotting were used to detect KISS-1 and matrix metalloproteinase-9 (MMP-9) expression in transfected cells.The growth of transfected cells was investigated by 3-(4,5-dimethylthiazolyl-2)-2,5-diphenyltetrazolium bromide (MTT) proliferation assays,and the cells' invasive potential was analyzed by basement membrane (Matrigel) invasion assays.The anti-tumor effects of KISS-1 were tested in vivo using allografts in nude mice.RESULTS:The expression level of KISS-1 mRNA and protein in BGC-823/KISS-1 transfected cells were significantly higher than in BGC-823/pcDNA3.1 transfected cells (P ＜ 0.05) or the parental BGC-823 cell line (P ＜0.05).The expression level of MMP-9 mRNA and protein in BGC-823/KISS-1 were significantly less than in BGC-823/pcDNA3.1 (P ＜ 0.05) or BGC-823 cells (P ＜0.05).MlT growth assays show the proliferation of BGC-823/KISS-1 cells at 48 h (0.642 ± 0.130) and 72h (0.530 ± 0.164) were significantly reduced compared to BGC-823/pcDNA3.1 (0.750 ± 0.163,0.645 ± 0.140)(P ＜ 0.05) and BGC-823 cells (0.782 ± 0.137,0.685 ±0.111) (P ＜ 0.05).Invasion assays indicate the invasive potential of BGC-823/KISS-1 cells (16.50 ± 14.88) is significantly reduced compared to BGC-823/pcDNA3.1(20.22 ± 14.87) (P ＜ 0.05) and BGC-823 cells after 24h (22.12 ± 16.12) (P ＜ 0.05).In vivo studies demonstrate the rate of pcDNA3.1-KISS-1 tumor growth is significantly slower than pcDNA3.1 and control cell tumor growth in nude mice.Furthermore,tumor volume of pcDNA3.1-KISS-1 tumors (939.38 ± 82.08 mm3) was significantly less than pcDNA3.1 (1250.46±44.36 mm3)and control tumors (1284.36 ± 55.26 mm3) (P ＜ 0.05).Moreover,the tumor mass of pcDNA3.1-KISS-1 tumors (0.494 ± 0.84 g) was significantly less than pcDNA3.1(0.668 ± 0.55 g) and control tumors (0.682 ± 0.38 g) (P＜ 0.05).CONCLUSION:KISS-1 may inhibit the proliferation and invasion of gastric carcinoma cells in vitro and in vivo through the downregulation of MMP-9.
NETL
NSTL
万方数据
维普
