首页|siRNA targeting of Cdx2 inhibits growth of human gastric cancer MGC-803 cells

siRNA targeting of Cdx2 inhibits growth of human gastric cancer MGC-803 cells

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AIM:To investigate the effects of small interference RNA (siRNA) targeting of Cdx2 on human gastric cancer MGC-803 cells in vitro and in vivo.METHODS:The recombinant pSilencer 4.1-Cdx2 siRNA plasmids were constructed and transfected into gastric cancer MGC-803 cells in vitro.The stable transfectants were selected.The effects of Cdx2 siRNA on growth,proliferation,cell cycle,apoptosis,migration and invasiveness of human gastric cancer MGC-803 cells were evaluated and the expression of phosphatase and tensin homolog (PTEN),caspase-9 and caspase-3 was observed in vitro by reverse transcription polymerase chain reaction (RT-PCR) and Western blotting analysis.We also investigated the effect of Cdx2 siRNA on growth of MGC-803 cells in nude mice in vivo.RESULTS:Cdx2 siRNA led to inhibition of endogenous Cdx2 mRNA and protein expression as determined by RT-PCR and Western blotting analysis.Cdx2 siRNA significantly inhibited cell growth and proliferation,blocked entry into the S-phase of the cell cycle,induced cell apoptosis,and reduced the motility and invasion of MGC-803 cells.Cdx2 siRNA also increased PTEN expression,and activated caspase-9 and caspase-3 in MGC-803 cells in vitro.In addition,siRNA targeting of Cdx2 inhibited the growth of MGC-803 cells and promoted tumor cell apoptosis in vivo in nude mice tumor models.CONCLUSION:Cdx2 was involved in regulating progression of human gastric cancer cells MGC-803.Manipulation of Cdx2 expression may be a potential therapeutic strategy for gastric cancer.

Cdx2Gastric cancerGrowthSmall interference RNA

Xiao-Tong Wang、Yu-Bo Xie、Qiang xiao

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Department of Surgery, The First Affiliated Hospital, Guangxi Medical University, Nanning 530021, Guangxi Zhuang Autonomous Region, China

Department of Anesthesiology, The First Affiliated Hospital, Guangxi Medical University, Nanning 530021,Gnangxi Zhuang Autonomous Region, China

Natural Science Foundation of ChinaNatural Science Foundation of ChinaHigher School Specialized Research Foundation for the Doctoral Program of ChinaPostdoctoral Science Foundation of ChinaNatural Science Foundation of Guangxi

81060201No.81060277201145031100022010033422011GXNSFA018273

2012

10.3748/wjg.v18.i16.1903

世界胃肠病学杂志(英文版)
太原消化病研治中心

世界胃肠病学杂志(英文版)

SCI
影响因子：1.001
ISSN：1007-9327
年,卷(期)：2012.18(16)
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