首页|Affinity peptide developed by phage display selection for targeting gastric cancer

Affinity peptide developed by phage display selection for targeting gastric cancer

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AIM:To develop an affinity peptide that binds to gastric cancer used for the detection of early gastric cancer.METHODS:A peptide screen was performed by biopanning the PhD-12 phage display library,clearing non-specific binders against tumor-adjacent normal appearing gastric mucosa and obtaining selective binding against freshly harvested gastric cancer tissues.Tumortargeted binding of selected peptides was confirmed by bound phage counts,enzyme-linked immunosorbent assay,competitive inhibition,fluorescence microscopy and semi-quantitative analysis on immunohistochemistry using different types of cancer tissues.RESULTS:Approximately 92.8％ of the non-specific phage clones were subtracted from the original phage library after two rounds of biopanning against normalappearing gastric mucosa.After the third round of positive screening,the peptide sequence AADNAKTKSFPV (AAD) appeared in 25％ (12/48) of the analyzed phages.For the control peptide,these values were 6.8 ± 2.3,5.1 ± 1.7,3.5 ± 2.1,4.6 ± 1.9 and 1.1 ± 0.5,respectively.The values for AAD peptide were statistically significant (P ＜ 0.01) for gastric cancer as compared with other histological classifications and control peptide.CONCLUSION:A novel peptide is discovered to have a specific binding activity to gastric cancer,and can be used to distinguish neoplastic from normal gastric mucosa,demonstrating the potential for early cancer detection on endoscopy.

Gastric cancerPeptidePhage libraryMolecular imagingEarly detectionImmunohistochemistryEnzyme-linked immunosorbent assay

Wen-Jie Zhang、Yan-Xia Sui、Arun Budha、Jian-Bao Zheng、Xue-Jun Sun、Ying-Chun Hou、Thomas D Wang、Shao-Ying Lu

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Department of General Surgery,The First Affiliated Hospital of Medical School, Xian Jiaotong University, Xi'an 710061, Shaanxi Province, China

The Third Hospital of Chengdu, Chengdu 610031, Sichuan Province, China

Shaanxi Normal University, Xi'an 710061,Shaanxi Province, China

Department of Medicine, Division of Gastroenterology and Hepatology, School of Medicine, University of Michigan, Ann Arbor, MI 48109, United States

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国家自然科学基金

81172359

2012

10.3748/wjg.v18.i17.2053

世界胃肠病学杂志(英文版)
太原消化病研治中心

世界胃肠病学杂志(英文版)

SCI
影响因子：1.001
ISSN：1007-9327
年,卷(期)：2012.18(17)
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