首页|Agmatine induces gastric protection against ischemic injury by reducing vascular permeability in rats

Agmatine induces gastric protection against ischemic injury by reducing vascular permeability in rats

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AIM:To investigate the effect of administration of agmarine (AGM) on gastric protection against ischemia reperfusion (I/R)injury.METHODS:Three groups of rats (6/group); sham,gastric I/R injury,and gastric I/R + AGM (100 mg/kg,i.p.given 15 min prior to gastric ischemia) were recruited.Gastric injury was conducted by ligating celiac artery for 30 rmin and reperfusion for another 30 min.Gastric tissues were histologically studied and immunostained with angiopoietin 1 (Ang-1) and Ang-2.Vascular endothelial growth factor (VEGF) and monocyte chemoattractant protein-1 (MCP-1) were measured in gastric tissue homogenate.To assess whether AKt/phosphatidyl inositol-3-kinase (PI3K) mediated the effect of AGM,an additional group was pretreated with Wortmannin (WM) (inhibitor of Akt/PI3K,15 μg/kg,i.p.),prior to ischemic injury and AGM treatment,and examined histologically and immunostained.Another set of experiments was run to study vascular permeability of the stomach using Evan's blue dye.RESULTS:AGM markedly reduced Evan's blue dye extravasation (3.58 ± 0.975 μg/stomach vs 1.175 ±0.374 μg/stomach,P ＜ 0.05),and VEGF (36.87 ± 2.71 pg/100 mg protein vs 48.4 ± 6.53 pg/100 mg protein,P ＜ 0.05) and MCP-1 tissue level (29.5 ± 7 pg/100 mg protein vs 41.17 ± 10.4 pg/100 mg protein,P ＜ 0.01).It preserved gastric histology and reduced congestion.Ang-1 and Ang-2 immunostaining were reduced in stomach sections of AGM-treated animals.The administration of WM abolished the protective effects of AGM and extensive hemorrhage and ulcerations were seen.CONCLUSION:AGM protects the stomach against I/R injury by reducing vascular permeability and inflammation.This protection is possibly mediated by Akt/PI3K.

Ischemia reperfusion injuryAgmatineWortmanninVascular permeabilityMonocyte chemoattractant protein-1StomachVascular endothelial growth factor

Abeer A Al Masri、Eman El Eter

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Department of Physiology,College of Medicine and King Khalid University Hospital, King Saud University, Rivadh 11461, Saudi Arabia

Deanship of Scientific Research at King Saud University,through research group projectCardiovascular research Group

RGPVPP-016

2012

10.3748/wjg.v18.i18.2188

世界胃肠病学杂志(英文版)
太原消化病研治中心

世界胃肠病学杂志(英文版)

SCI
影响因子：1.001
ISSN：1007-9327
年,卷(期)：2012.18(18)
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