首页|Hexahydrocurcumin enhances inhibitory effect of 5-fluorouracil on HT-29 human colon cancer cells

Hexahydrocurcumin enhances inhibitory effect of 5-fluorouracil on HT-29 human colon cancer cells

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AIM:To investigate the ability of hexahydrocurcumin (HHC) to enhance 5-fluorouracil (5-FU) in inhibiting the growth of HT-29 cells by focusing on cyclooxygenase (COX)-2 expression.METHODS:Antiproliferative effects of HHC and 5-FU,alone and in combination,on growth of HT-29 human colon cancer cells were assessed using 5-diphenyltetrazolium bromide (MTT) reduction assay.In combination treatment,low doses of 5-FU were used combined with various concentrations of HHC to minimize the toxicity and side effects of 5-FU.The therapeutic effects of these drugs on down-regulation of COX-2 mRNA and protein expression were examined using semi-quantitative reverse transcription-polymerase chain reaction (RT-PCR) and Western blotting analysis.RESULTS:MTT reduction assay indicated that HHC alone markedly decreased the viability of HT-29 human colon cancer cells compared to control.Semi-quantitative RT-PCR analysis indicated that HHC is a selective COX-2 inhibitor.This finding was supported by the observation that HHC significantly down-regulates COX-2 mRNA expression compared to the control (control:100.05％ ± 0.03％ vs HHC:61.01％ ± 0.35％,P ＜ 0.05)but does not alter COX-1 mRNA.In combined treatment,addition of HHC to a low dose of 5-FU exerts a synergistic effect against the growth of HT-29 cells by markedly reducing cell viability to a greater degree than monotherapy.Semi-quantitative RT-PCR indicated that 5-FU at the concentration of 5 μmol/L in combination with HHC at the concentration of 25 μmol/L significantly down-regulates COX-2 mRNA expression when compared with values in cells treated with 5-FU or HHC alone (HHC + 5-FU:31.93％ ± 5.69％,5-FU:100.66％± 4.52％ vs HHC:61.01％ ± 0.35％,P ＜ 0.05).CONCLUSION:HHC together with 5-FU exerts a synergistic effect and may prove chemotherapeutically useful in treating human colon cancer.

Colon cancerHexahydrocurcumin5-FluorouracilCombination treatmentCyclooxygenase-2Synergistic effect

Khanitta Srimuangwong、Chainarong Tocharus、Pornphrom Yoysungnoen Chintana、Apichart Suksamrarn、Jiraporn Tocharus

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Department of Anatomy, Faculty of Medical Science, Naresuan University, Phitsanulok 65000,Thailand

Department of Anatomy, Faculty of Medicine, Chiang Mai University, Chiang Mai 50200, Thailand

Division of Physiology,Faculty of Medicine, Thammasat University, Rangsit Campus,Pathumthani 12120, Thailand

Department of Chemistry, Faculty of Science, Ramkhamhaeng University, Bangkok 10240, Thailand

Department of Physiology, Faculty of Medicine, Chiang Mai University, Chiang Mai 50200, Thailand

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2012

10.3748/wjg.v18.i19.2383

世界胃肠病学杂志(英文版)
太原消化病研治中心

世界胃肠病学杂志(英文版)

SCI
影响因子：1.001
ISSN：1007-9327
年,卷(期)：2012.18(19)
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