首页|Glypican-3 expression and its relationship with recurrence of HCC after liver transplantation
Glypican-3 expression and its relationship with recurrence of HCC after liver transplantation
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NETL
NSTL
万方数据
维普
AIM:To investigate the diagnostic value of glypican-3 (GPC3) and its relationship with hepatocellular carcinoma (HCC) recurrence after liver transplantation.METHODS:HCC tissue samples (n =31) obtained from patients who had undergone liver transplantation were analyzed.GPC3 mRNA and protein expression were analyzed by TaqMan real-time reverse transcription-polymerase chain reaction and immunohistochemistry.Correlation between the GPC3 expression and clinicopathological features was analyzed.The potential prognostic value of GPC3 was investigated by comparing recurrence-free survival between HCC patients with and without GPC3 expression.RESULTS:Using a cutoff value of 3.5 × 10-2,20 of 31 cancerous tissues had expression values of ＞ 3.5 ×10-2,whereas 3 of 31 adjacent non-neoplastic parenchyma and 0 of 20 control liver tissues had expression values of ＞ 3.5 × 10-2 (P ＜ 0.001).GPC3 protein was immunoexpressed in 68％ of cancerous tissues,but not in adjacent non-neoplastic parenchyma and control liver tissues.Vascular invasion was significantly related to GPC3 expression (P ＜ 0.05).Recurrence-free survival was significantly longer for patients without GPC3 mRNA overexpression (＞ 3.5 × 102) and those without vascular invasion (P ＜ 0.05 for both).CONCLUSION:GPC3 expression may serve as a valuable diagnostic marker for HCC.GPC3 mRNA overexpression may be an adverse indicator for HCC patients after liver transplantation.
Department of Transplantation Surgery,Tianjin First Central Hospital, Key Laboratory for Critical Care Medicine of the Ministry of Health, Tianjin 300192, China
Department of Immunology, Tianjin Medical University, Tianjin 300070, China
NETL
NSTL
万方数据
维普
