首页|Key factors in developing the trinitrobenzene sulfonic acid-induced post-inflammatory irritable bowel syndrome model in rats
Key factors in developing the trinitrobenzene sulfonic acid-induced post-inflammatory irritable bowel syndrome model in rats
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NETL
NSTL
万方数据
维普
AIM:To investigate the key factors in developing the trinitrobenzene sulfonic acid (TNBS)-induced postinflammatory irritable bowel syndrome (PI-IBS) model in rats.METHODS:TNBS was administered to rats at the following conditions:(1) with different doses (20,10,5mg/0.8 mL per rat); (2) with same dose in different concentrations (20 mg/rat,25,50 mg/mL); (3) in different ethanol percentage (25％,50％); and (4) at depth either 4 cm or 8 cm from anus.At 5 d and 4 wk after TNBS administration,inflammation severity and inflammation resolution were evaluated.At 4 and 8 wk after TNBS application,visceral hyperalgesia and enterochromaffin (EC) cell hyperplasia were assayed by abdominal withdrawal reflex test,silver staining and capillary electrophoresis.RESULTS:Our results showed that:(1) TNBS induced dose-dependent acute inflammation and inflammation resolution.At 5 d post TNBS,the pathological score and myeloperoxidase (MPO) activity in all TNBS treated rats were significantly elevated compared to that of the control (9.48 ± 1.86,8.18 ± 0.67,5.78 ±0.77 vs 0,and 3.55 ± 1.11,1.80 ± 0.82,0.97 ± 0.08unit/mg vs 0.14 ± 0.01 unit/mg,P ＜ 0.05).At 4 wk post TNBS,the pathological score in high and median dose TNBS-treated rats were still significantly higher than that of the control (1.52 ± 0.38 and 0.80 ± 0.35vs 0,P ＜ 0.05); (2) Intracolonic TNBS administration position affected the persistence of visceral hyperalgesia.At 4 wk post TNBS,abdominal withdrawal reflex (AWR) threshold pressure in all TNBS-treated groups were decreased compared to that of the control (21.52± 1.73 and 27.10 ± 1.94 mmHg vs 34.44 ± 1.89mmHg,P ＜ 0.05).At 8 wk post TNBS,AWR threshold pressure in 8 cm administration group was still significantly decreased (23.33 ± 1.33 mmHg vs 36.79 ± 2.29mmHg,P ＜ 0.05); (3) Ethanol percentage affected the TNBS-induced inflammation severity and visceral hyperalgesia.In TNBS-25％ ethanol-treated group,the pathological score and MPO activity were significantly lowered compared to that of the TNBS-50％ ethanoltreated group,while AWR threshold pressure were significantly elevated (36.33 ± 0.61 mmHg vs 23.33 ± 1.33mmHg,P ＜ 0.05); and (4) TNBS (5 mg/0.8 mL per rat,in 50％ ethanol,8 cm from anus)-treated rats recovered completely from the inflammation with acquired visceral hyperalgesia and EC cell hyperplasia at 4 wk after TNBS administration.CONCLUSION:TNBS dosage,concentration,intracoIonic administration position,and ethanol percentage play important roles in developing visceral hyperalgesia and EC cell hyperplasia of TNBS-induced PI-IBS rats.
NETL
NSTL
万方数据
维普
