首页|Effects of anesthetic methods on preserving anti-tumor T-helper polarization following hepatectomy

Effects of anesthetic methods on preserving anti-tumor T-helper polarization following hepatectomy

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AIM:To investigate the impact of different anesthetic techniques on T-helper (Th) cell subsets in hepatocellular carcinoma (HCC) patients undergoing hepatectomy.METHODS:Sixty-one HCC patients who received hepatectomies were randomized into an epidural combined general anesthesia (G + E; n =31) or a general anesthesia (G; n =30) group.Blood samples were obtained the morning before the operation (d0),and on the second (d2) and seventh (d7) day after the operation.Th cell contents were evaluated using flow cytometry,realtime reverse transcription-polymerase chain reaction and enzyme-linked immunosorbent assay.RESULTS:In all 61 patients,Th1 and Th2 cell frequencies,and interferon-γ (IFN-γ) mRNA expression markedly increased on d2,compared to d0.They recovered slightly on d7,and the Th1/Th2 ratio increased markedly on d7,compared with d2.In contrast,Th17,regulatory T cell (Treg),and interieukin-17 (IL-17) levels and FOXP3 mRNA expression showed no significant change on d2,and then markedly decreased on d7.Similarly,plasma IFN-γ concentration on d2 was much higher than that on d0,and then partly recovered on d7.As compared with the G group,in the G + E group,Th1 cell frequencies and the Th1/Th2 ratio were slightly higher on d2 and significantly higher on d7,while Th2,Th17,and Treg cell frequencies were slightly lower on d2,and significantly lower on d7.Consistently,on d7,IFN-γ mRNA and protein levels and the IFN-γ/IL-4 ratio in the G + E group were higher than those in the G group.In contrast,the IL-17 mRNA level,and IL-17 and transforming growth factor-β1 concentrations in the G + E group were lower than those in the G group.CONCLUSION:G + E is superior to G in shifting the Th1/Th2 balance towards Th1,while decreasing Th17 and Treg,potentially benefiting HCC patients by promoting anti-tumor Th polarization.

Epidural anesthesiaGeneral anesthesiaHepatocellular carcinomaT-helper cellTumor resection

Di Zhou、Fang-Ming Gu、Qiang Gao、Quan-Lin Li、Jian Zhou、Chang-Hong Miao

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Department of Anesthesiology, Zhongshan Hospital,Fudan University, Shanghai 200032, China

Liver Cancer Institute, Zhongshan Hospital, Fudan University, Shanghai 200032, China

the Third Department of Hepatic Surgery, Eastern Hepatobiliary Surgery Hospital, Second Military Medical University, Shanghai 200438, China

Institute of Biomedical Sciences, Fudan University,Shanghai 200032, China

Department of Anesthesiology, Fudan University Shanghai Cancer Center, Shanghai 200032, China

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State 863 High Technology R and D Project of China国家自然科学基金Shanghai Rising-Star ProgramFANEDD上海市科委资助项目Shanghai "Chen Guang" Project

2007AA02Z4793097294910QA1401300201183114119a440011CG02

2012

10.3748/wjg.v18.24.3089

世界胃肠病学杂志(英文版)
太原消化病研治中心

世界胃肠病学杂志(英文版)

SCI
影响因子：1.001
ISSN：1007-9327
年,卷(期)：2012.18(24)
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