首页|Knockdown of liver-intestine cadherin decreases BGC823 cell invasiveness and metastasis in vivo

Knockdown of liver-intestine cadherin decreases BGC823 cell invasiveness and metastasis in vivo

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AIM:To assess BGC823 gastric cancer (GC) cell metastasis after knockdown of liver-intestine cadherin (CDH17) and the therapeutic value of CDH17-RNAilentivirus in vivo.METHODS:We evaluated primary tumor growth and assessed local infiltration and systemic tumor dissemination using an orthotopic implantation technique.The therapeutic value of CDH17 knockdown was examined by intratumoral administration of CDH17-RNA interference (RNAi)-lentivirus in an established GC tumor xenograft mouse model.Furthermore,a comparative proteomic approach was utilized to identify differentially expressed proteins in BGC823 and lenti-CDH17-miRneg cells following CDH17 knockdown.RESULTS:Metastases in the liver and lung appeared earlier and more frequently in animals with tumors derived from BGC823 or lenti-CDH17-miR-neg cells than in tumors derived from lenti-CDH17-miR-B cells.Average tumor weight and volume in the CDH17-RNAi-lentivirus-treated group were significantly lower than those in the control group (tumor volume:0.89 ± 0.04 cm3 vs 1.16 ± 0.06 cm3,P ＜ 0.05; tumor weight:1.15 ±0.58 g vs 2.09 ± 0.08 g,P ＜ 0.05).Fifteen differentially expressed proteins were identified after CDH17 silencing in BGC823 cells,including a variety of cytoskeletal and chaperone proteins as well as proteins involved in metabolism,immunity/defense,cell proliferation and differentiation,cell cycle,and signal transduction.CONCLUSION:Our data establish a foundation for future studies of the comprehensive protein expression patterns and effects of CDH17 in GC.

CadherinGastric cancerIntratumoral administrationLiverOrthotopic implantationProteomics

YU Xu、Jin Zhang、Qi-Sheng Liu、Wei-Guo Dong

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Department of Otolaryngology, Renmin Hospital of Wuhan University, Wuhan 430060, Hubei Province, China

Department of Gastroenterology, Renmin Hospital of Wuhan University, Wuhan 430060, Hubei Province, China

Department of Gastroenterology, Central Hospital of Xianning City, Xianning 437000, Hubei Province, China

国家自然科学基金

30871147

2012

10.3748/wjg.v18.i24.3129

世界胃肠病学杂志(英文版)
太原消化病研治中心

世界胃肠病学杂志(英文版)

SCI
影响因子：1.001
ISSN：1007-9327
年,卷(期)：2012.18(24)
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