首页|Intestinal alkaline phosphatase in the colonic mucosa of children with inflammatory bowel disease

Intestinal alkaline phosphatase in the colonic mucosa of children with inflammatory bowel disease

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AIM:To investigate intestinal alkaline phosphatase (iAP) in the intestinal mucosa of children with inflammatory bowel disease (IBD).METHODS:Colonic biopsy samples were taken from 15 newly diagnosed IBD patients and from 10 healthy controls.In IBD patients,specimens were obtained both from inflamed and non-inflamed areas.The iAP mRNA and protein expression was determined by reverse transcription-polymerase chain reaction and Western blotting analysis,respectively.Tissue localization of iAP and Toll-like receptor (TLR) 4 was investigated by immunofluorescent staining.RESULTS:The iAP protein level in the inflamed mucosa of children with Crohn's disease (CD) and ulcerative colitis (UC) was significantly decreased when compared with controls (both P ＜ 0.05).Similarly,we found a significantly decreased level of iAP protein in the inflamed mucosa in CD compared with non-inflamed mucosa in CD (P ＜ 0.05).In addition,the iAP protein level in inflamed colonic mucosa in patients with UC was decreased compared with non-inflamed mucosa in patients with CD (P ＜ 0.05).iAP protein levels in the non-inflamed mucosa of patients with CD were similar to controls.iAP mRNA expression in inflamed colonic mucosa of children with CD and UC was not significantly different from that in non-inflamed colonic mucosa with CD.Expression of iAP mRNA in patients with non-inflamed mucosa and in controls were similar.Co-localization of iAP with TLR4 showed intense staining with a dotted-like pattern.iAP was present in the inflamed and non-inflamed mucosa of patients with CD,UC,and in control biopsy specimens,irrespective of whether it was present in the terminal ileum or in the colon.However,the fluorescent signal of TLR4 was more pronounced in the colon compared with the terminal ileum in all groups studied.CONCLUSION:Lower than normal iAP protein levels in inflamed mucosa of IBD patients may indicate a role for iAP in inflammatory lesions in IBD.Based on our results,administration of exogenous iAP enzyme to patients with the active form of IBD may be a therapeutic option.

Intestinal alkaline phosphataseToll-like receptorColonic biopsyChildrenInflammatory bowel disease

Kriszta Molnár、(A)dám Vannay、Beáta Szebeni、Nóra Fanni Bánki、Erna Sziksz、(A)ron Cseh、Hajnalka Gy(o)rffy

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First Department of Pediatrics, Semmelweis University, H-1083 Budapest, Hungary

Research Group for Pediatrics and Nephrology, Semmelweis University and Hungarian Academy of Sciences, H-1083 Budapest, Hungary

SE-MTA "Lendulet" Diabetes Research Group, H-1083 Budapest, Hungary

Second Department of Pathology, Semmelweis University, H-1091 Budapest, Hungary

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Veres G and Vannay (A) are holders of the János Bolyai Research grantVeres G and Vannay (A) are holders of the János Bolyai Research grantVeres G and Vannay (A) are holders of the János Bolyai Research grantVeres G and Vannay (A) are holders of the János Bolyai Research grantJános Bolyai Research Scholarship of the Hungarian Academy of Sciences

OTKA-76316OTKA-K81117ETT-028-02

2012

10.3748/wjg.v18.i25.3254

世界胃肠病学杂志(英文版)
太原消化病研治中心

世界胃肠病学杂志(英文版)

SCI
影响因子：1.001
ISSN：1007-9327
年,卷(期)：2012.18(25)
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