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Acute pancreatitis in aging animals: Loss of pancreatitis-associated protein protection?

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AIM:To investigate the effect of age on severity of acute pancreatitis (AP) using biochemical markers,histology and expression of the protective pancreatitisassociated proteins (PAPs).METHODS:AP was induced via intraductal injection of 4％ sodium taurocholate in young and old rats.Sera and pancreata were assayed at 24 h for the parameters listed above; we also employed a novel molecular technique to assess bacterial infiltration using polymerase chain reaction to measure bacterial genomic ribosomal RNA.RESULTS:At 24 h after induction of AP,the pancreata of older animals had less edema (mean ± SE histologic score of young vs old:3.11 ± 0.16 vs 2.50 ±-0.11,P ＜0.05),decreased local inflammatory response (histologic score of stromal infiltrate:3.11 ± 0.27 vs 2.00 ± 0.17,P ＜ 0.05) and increased bacterial infiltration (174％ ±52％ increase from sham vs 377％ ± 4％,P ＜ 0.05).A decreased expression of PAP1 and PAP2 was demonstrated by Western blotting analysis and immunohistochemical staining.There were no differences in serum amylase and lipase activity,or tissue myeloperoxidase or monocyte chemotactic protein-1 levels.However,in the most-aged group,serum C-reactive protein levels were higher (young vs old:0.249 ± 0.04 mg/dL vs 2.45± 0.68 mg/dL,P ＜ 0.05).CONCLUSION:In older animals,there is depressed PAP expression related to a blunted inflammatory response in AP which is associated with worsened bacterial infiltration and higher C-reactive protein level; this may explain the more aggressive clinical course.

Acute pancreatitisAgingRatsPancreatitis-associated proteinMolecular biology

Sophia Fu、Albert Stanek、Cathy M Mueller、Nefertti A Brown、Chongmin Huan、Martin H Bluth、Michael E Zenilman

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Department of Surgery, SUNY Downstate Medical School, Brooklyn, NY 11203, United States

Department of Pathology, Wayne State University, Detroit, MI 48202, United States

Department of Surgery, Johns Hopkins School of Medicine and SUNY Downstate School of Public Health, Bethesda, MD 20814, United States

2012

10.3748/wjg.v18.i26.3379

世界胃肠病学杂志(英文版)
太原消化病研治中心

世界胃肠病学杂志(英文版)

SCI
影响因子：1.001
ISSN：1007-9327
年,卷(期)：2012.18(26)
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