首页|Analysis of hepcidin expression: In situ hybridization and quantitative polymerase chain reaction from paraffin sections

Analysis of hepcidin expression: In situ hybridization and quantitative polymerase chain reaction from paraffin sections

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AIM:TO establish methods for quantitative polymerase chain reaction (PCR) for hepcidin using RNAs isolated from paraffin-embedded sections and in situ hybridization of hepatocellular carcinoma (HCC).METHODS:Total RNA from paraffin-embedded sections was isolated from 68 paraffin-embedded samples of HCC.Samples came from 54 male and 14 female patients with a mean age of 66.8 ± 7.8 years.Quantitative PCR was performed.Immunohistochemistry and in situ hybridization for hepcidin were also performed.RESULTS:Quantitative PCR for hepcidin using RNAs isolated from paraffin-embedded sections of HCC was performed successfully.The expression level of hepcidin mRNA in cancer tissues was significantly higher than that in non-cancer tissues.A method of in situ hybridization for hepcidin was established successfully,and this demonstrated that hepcidin mRNA was expressed in non-cancerous tissue but absent in cancerous tissue.CONCLUSION:We have established novel methods for quantitative PCR for hepcidin using RNAs isolated from paraffin-embedded sections and in situ hybridization of HCC.

HepcidinExpressionIn situ hybridizationImmunohistochemistryReal-time polymerase chain reaction

Yuhki Sakuraoka、Tokihiko Sawada、Takayuki Shiraki、Kyunghwa Park、Yuhichiro Sakurai、Naohisa Tomosugi、Keiichi Kubota

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Second Department of Surgery, School of Medicine, Dokkyo Medical University, Kitakobayashi 880, Mibu, Shimotsuga, Tochigi 321-0293, Japan

Proteomics Research Unit, Division of Advanced Medicine, Medical Research Institute, Kanazawa Medical College, Daigaku 1-1, Uchinada, Kanazawa 920-0293,Japan

2012

10.3748/wjg.v18.i28.3727

世界胃肠病学杂志(英文版)
太原消化病研治中心

世界胃肠病学杂志(英文版)

SCI
影响因子：1.001
ISSN：1007-9327
年,卷(期)：2012.18(28)
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