首页|Evaluation of a novel hybrid bioartificial liver based on a multi-layer fiat-plate bioreactor
Evaluation of a novel hybrid bioartificial liver based on a multi-layer fiat-plate bioreactor
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AIM:TO evaluate the efficacy and safety of a hybrid bioartificial liver (HBAL) system in the treatment of acute liver failure.METHODS:Canine models with acute liver failure were introduced with intravenous administration of D-galactosamine.The animals were divided into:the HBAL treatment group (n =8),in which the canines received a 3-h treatment of HBAL; the bioartificial liver (BAL) treatment group (n =8),in which the canines received a 3-h treatment of BAL; the non-bioartificial liver (NBAL) treatment group (n =8),in which the canines received a 3-h treatment of NBAL; the control group (n =8),in which the canines received no additional treatment.Biochemical parameters and survival time were determined.Levels of xenoantibodies,RNA of porcine endogenous retrovirus (PERV) and reverse transcriptase (RT) activity in the plasma were detected.RESULTS:Biochemical parameters were significantly decreased in all treatment groups.The TBIL level in the HBAL group was lower than that in other groups (2.19 ± 0.55 μmol/L vs 24.2 ± 6.45 μmol/L,12.47 ± 3.62 μmol/L,3.77 ± 1.83 μmol/L,P ＜ 0.05).The prothrombin time (PT) in the BAL and HBAL groups was significantly shorter than the NBAL and control groups (18.47 ± 4.41 s,15.5 ± 1.56 s vs 28.67 ± 5.71 s,21.71 ± 3.4 s,P ＜ 0.05),and the PT in the HBAL group was shortest of all the groups.The albumin in the BAL and HBAL groups significantly increased and a significantly higher level was observed in the HBAL group compared with the BAL group (27.7 ± 1.7 g/L vs 25.24 ± 1.93 g/L).In the HBAL group,the ammonia levels significantly decreased from 54.37 ± 6.86 to 37.75 ± 6.09 after treatment (P ＜ 0.05); there were significant difference in ammonia levels between other the groups (P ＜ 0.05).The levels of antibodies were similar before and after treatment.The PERV RNA and the RT activity in the canine plasma were all negative.CONCLUSION:The HBAL showed great efficiency and safety in the treatment of acute liver failure.
Xiao-Lei Shi、Yue Zhang、Xue-Hui Chu、Bing Han、Jin-Yang Gu、Jiang-Qiang xiao、Jia-Jun Tan
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Department of Hepatobiliary Surgery,Jiangsu Province's Key Medical Center for Hepatobiliary Disease, The Affiliated Drum Tower Hospital of Nanjing University Medical School, Nanjing 210008, Jiangsu Province, China
A grant from the National Natural Science Foundation of China
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