首页|Non-invasive determination of hepatic steatosis by acoustic structure quantification from ultrasound echo amplitude
Non-invasive determination of hepatic steatosis by acoustic structure quantification from ultrasound echo amplitude
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NETL
NSTL
万方数据
维普
AIM:To use leptin-deficient (ob/ob) mice with demonstrated differences in steatosis levels to test a new diagnostic method using the acoustical structure quantification (ASQ) mode and the associated analytical parameter,"focal disturbance ratio" (FD-ratio).METHODS:Nine ob/ob mice,at 5,8,and 12 wk of age (n =3 in each age group),were used as models for hepatic steatosis.Echo signals obtained from ultrasonography in the mice were analyzed by ASQ,which uses a statistical analysis of echo amplitude to estimate inhomogeneity in the diagnostic region.FD-ratio,as calculated from this analysis,was the focus of the present study.FD-ratio and fat droplet areas and sizes were compared between age groups.RESULTS:No fibrosis or inflammation was observed in any of the groups.The fat droplet area significantly (P ＜ 0.01) increased with age from 1.25％ ± 0.28％ at 5 wk to 31.07％ ± 0.48％ at 8 wk to 51.69％ ± 3.19％at 12 wk.The median fat droplet size also significantly (P ＜ 0.01) increased with age,from 1.33 (0.55-10.52)μm at 5 wk,2.82 (0.61-44.13) μm at 8 wk and 6.34(0.66-81.83) μm at 12 wk.The mean FD-ratio was 0.42± 0.11 at 5 wk,0.11 ± 0.05 at 8 wk,and 0.03 ± 0.02at 12 wk.The FD-ratio was significantly lower at 12 wk than at 5 wk and 8 wk (P ＜ 0.01).A significant negative correlation was observed between the FD-ratio and either the fat droplet area (r =-0.7211,P =0.0017) or fat droplet size (r =-0.9811,P =0.0052).CONCLUSION:This tool for statistical analysis of signals from ultrasonography using the FD-ratio can be used to accurately quantify fat in vivo in an animal model of hepatic steatosis,and may serve as a quantitative biomarker of hepatic steatosis.
NETL
NSTL
万方数据
维普
