首页|Effects of Lactobacillus plantarum on gut barrier function in experimental obstructive jaundice
Effects of Lactobacillus plantarum on gut barrier function in experimental obstructive jaundice
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NETL
NSTL
万方数据
维普
AIM:To investigate the mechanisms of Lactobacillus plantarum (L.plantarum) action on gut barrier in preoperative and postoperative experimental obstructive jaundice in rats.METHODS:Forty rats were randomly divided into groups of sham-operation,bile duct ligation (BDL),BDL + L.plantarum,BDL + internal biliary drainage (IBD),and BDL + IBD + L.plantarum.Ten days after L,plantarum administration,blood and ileal samples were collected from the rats for morphological examination,and intestinal barrier function,liver function,intestinal oxidative stress and protein kinase C (PKC) activity measurement.The distribution and expression of the PKC and tight junction (TJ) proteins,such as occludin,zonula occludens-1,claudin-1,claudin-4,junction adhesion molecule-A and F-actin,were examined by confocal laser scanning microscopy,immunohistochemistry,Western blotting,real-time fluorescent quantitative polymerase chain reaction assay.RESULTS:L.plantarum administration substantially restored gut barrier,decreased enterocyte apoptosis,improved intestinal oxidative stress,promoted the activity and expression of protein kinase (BDL vs BDL + L.plantarum,0.295 ± 0.007 vs 0.349 ± 0.003,P ＜ 0.05;BDL + IBD vs BDL + IBD + L.plantarum,0.407 ± 0.046 vs 0.465 ± 0.135,P ＜ 0.05),and particularly enhanced the expression and phosphorylation of TJ proteins in the experimental obstructive jaundice (BDL vs BDL + L.plantarum,0.266 ± 0.118 vs 0.326 ± 0.009,P ＜ 0.05).The protective effect of L.plantarum was more prominent after internal biliary drainage (BDL + IBD vs BDL + IBD + L.plantarum,0.415 ± 0.105 vS 0.494 ± 0.145,P ＜ 0.05).CONCLUSION:L.plantarum can decrease intestinal epithelial cell apoptosis,reduce oxidative stress,and prevent TJ disruption in biliary obstruction by activating the PKC pathway.
NETL
NSTL
万方数据
维普
