首页|Overexpression of the M2 isoform of pyruvate kinase is an adverse prognostic factor for signet ring cell gastric cancer

Overexpression of the M2 isoform of pyruvate kinase is an adverse prognostic factor for signet ring cell gastric cancer

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AIM:To investigate M2 isoform of pyruvate kinase (PKM2) expression in gastric cancers and evaluate its potential as a prognostic biomarker and an anticancer target.METHODS:All tissue samples were derived from gastric cancer patients underwent curative gastrectomy as a primary treatment.Clinical and pathological information were obtained from the medical records.Gene expression microarray data from 60 cancer and 19 noncancer gastric tissues were analyzed to evaluate the expression level of PKM2 mRNA.Tissue microarrays were constructed from 368 gastric cancer patients.Immunohistochemistry was used to measure PKM2 expression and PKM2 positivity of cancer was determined by proportion of PKM2-positive tumor cells and staining intensity.Association between PKM2 expression and the clinicopathological factors was evaluated and the correlation between PKM2 and cancer prognosis was evaluated.RESULTS:PKM2 mRNA levels were increased more than 2-fold in primary gastric cancers compared to adjacent normal tissues from the same patients (log transformed expression level:7.6 ± 0.65 vs 6.3 ± 0.51,P ＜ 0.001).Moreover,differentiated type cancers had significantly higher PKM2 mRNA compared to undifferentiated type cancers (log transformed expression level:7.8 ± 0.70 vs 6.7 ± 0.71,P ＜ 0.001).PKM2 prorein was mainly localized in the cytoplasm of primary cancer cells and detected in 144 of 368 (39.1％) human gastric cancer cases.PKM2 expression was not related with stage (P =0.811),but strongly correlated with gastric cancer differentiation (P ＜ 0.001).Differentiated type cancers expressed more PKM2 protein than did the undifferentiated ones.Well differentiated adenocarcinoma showed 63.6％ PKM2-positive cells; in contrast,signet-ring cell cancers showed only 17.7％ PKM2-positive cells.Importantly,PKM2 expression was correlated with shorter overall survival (P ＜ 0.05) independent of stage only in signet-ring cell cancers.CONCLUSION:PKM2 expression might be an adverse prognostic factor for signet-ring cell carcinomas.Its function and potential as a prognostic marker should be further verified in gastric cancer.

Gastric cancerM2 isoform of pyruvate kinaseBiomarkerSignet ring cell carcinomaPrognosis

Jae Yun Lim、Sun Och Yoon、So Young Seol、Soon Won Hong、Jong Won Kim、Seung Ho Choi、Jae Yong Cho

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Department of Medical Oncology, Gangnam Severance Cancer Hospital,Yonsei University College of Medicine, Seoul 135-720, South Korea

Department of Pathology,Gangnam Severance Cancer Hospital, Yonsei University College of Medicine, Seoul 135-720, South Korea

Department of Surgery,Gangnam Severance Cancer Hospital, Yonsei University College of Medicine, Seoul 135-720, South Korea

Faculty Research Grant of Yonsei University College of Medicine for 2011Faculty Research Grant of Yonsei University College of Medicine for 2011A Faculty Research Grant of Department of Internal Medicine,Yonsei University College of Medicine for 2010Basic Science Research Program through the National Research Foundation of Korea funded by the Ministry of Education,Science and

6-2011-01136-2011-01462010-0024248

2012

10.3748/wjg.v18.i30.4037

世界胃肠病学杂志(英文版)
太原消化病研治中心

世界胃肠病学杂志(英文版)

SCI
影响因子：1.001
ISSN：1007-9327
年,卷(期)：2012.18(30)
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