首页|Growth inhibitory effects of Phyllanthus niruri extracts in combination with cisplatin on cancer cell lines

Growth inhibitory effects of Phyllanthus niruri extracts in combination with cisplatin on cancer cell lines

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AIM:To investigate the cytotoxic effects of spray-dried extracts of Phyllanthus niruri in combination with cisplatin on two cancer cell lines.METHODS:Colorectal carcinoma (HT29) and human hepatocellular carcinoma (HepG2) cells were treated with spray-dried extracts of Phy//anthus niruri (SDEPN) either alone or in combination with cisplatin at different concentrations (0.5 mg/mL and 1 mg/mL) for 4 h and 24 h.To verify and quantify cancer cells treated with these products as well as identify the cell cycle stage and cell viability,we stained the cells with propidium iodide and assessed them by flow cytometry.The percentage of cells in different cell cycle phases was quantified and data were expressed as histograms.Significant differences between groups were determined using analysis of variance and Bonferroni's test,as indicated.A value of P ＜ 0.05 was considered to be statistically significant.RESULTS:SDEPN had significantly different cytotoxic effects on HT29 (2.81 ± 0.11 vs 3.51 ± 1.13,P ＞ 0.05) and HepG2 (5.07 ± 0.3 vs 15.9 ± 1.04,P ＜0.001) cells when compared to control cells for 4 h.SDEPN also had significantly different cytotoxic effects on HT29 (1.91 ± 0.57 vs 4.53 ± 1.22,P ＞ 0.05) and HepG2 (14.56 ± 1.6 vs 35.67 ± 3.94,P ＜ 0.001) cells when compared to control cells for 24 h.Both cell lines were killed by cisplatin in a dose-dependent manner compared to control cells (HepG2 cells for 4 h:10.78 ± 1.58 vs 53.89 ± 1.53,P ＜ 0.001; 24 h:8.9 ± 1.43 vs 62.78 ± 1.87,P ＜ 0.001 and HT29 cells for 4 h:9.52 ± 0.913 vs 49.86 ± 2.89,P ＜ 0.001; 24 h:11.78 ± 1.05 vs 53.34 ± 2.65,P ＜ 0.001).In HT29 cells,pretreatment with SDEPN and subsequent treatment with cisplatin resulted in a greater number of cells being killed (12.78 ± 1.01 vs 93.76 ± 1.6,P ＜ 0.001).HepG2 cells showed significant cell killing with treatment with SDEPN when combined with cisplatin (12.87±2.78 vs 78.8 ± 3.02,P ＜ 0.001).CONCLUSION:SDEPN is selectively toxic against two cancer cell lines.Moreover,SDEPN in combination with cisplatin induces a synergistic increase in the cell death of both HT29 and HepG2 cells.

CisplatinColorectal cancerLiver cancerPhyllanthus niruriCytotoxic effect

Raimundo Fernandes de Araújo Júnior、Luiz Alberto Lira Soares、Cínthia Raquel da Costa Porto、Ranniere Gurgel Furtado de Aquino、Hugo Gon(c)alo Guedes、Pedro Ros Petrovick、Tatiane Pereira de Souza

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Department of Morphology/Postgraduate Program in Morphological Sciences,Federal University of Rio Grande do Norte, Natal 59072-970,Rio Grande do Norte, Brazil

Postgraduate Program in Pharmaceutical Sciences, Federal University of Pernambuco, Recife 50670-901, Pernambuco, Brazil

Postgraduate Program in Pharmaceutical Sciences, Federal University of Rio Grande do Norte, Natal 59012-570, Rio Grande do Norte, Brazil

Department of Production and control of drugs, Federal University of Rio Grande do Sul, Porto Alegre 90610-000, Rio Grande do Sul, Brazil

Department of Food and Drug Administration, Federal University of Amazonas, Manaus 69077-000,Amazonas, Brazil

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Conselho Nacional de Desenvolvimento Cientifico e Tecnológico (CNPq)financial support and Postgraduate Program in Pharmaceutical Sciences,Federal University of Rio Grande do Norte

CNPq470179/2009-0

2012

10.3748/wjg.v18.i31.4162

世界胃肠病学杂志(英文版)
太原消化病研治中心

世界胃肠病学杂志(英文版)

SCI
影响因子：1.001
ISSN：1007-9327
年,卷(期)：2012.18(31)
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