首页|Use of butyrate or glutamine in enema solution reduces inflammation and fibrosis in experimental diversion colitis

Use of butyrate or glutamine in enema solution reduces inflammation and fibrosis in experimental diversion colitis

扫码查看
原文链接
  • NETL
  • NSTL
  • 万方数据
  • 维普
AIM:To investigate whether butyrate or glutamine enemas could diminish inflammation in experimental diversion colitis.METHODS:Wistar specific pathogen-free rats were submitted to a Hartmann's end colostomy and treated with enemas containing glutamine,butyrate,or saline.Enemas were administered twice a week in the excluded segment of the colon from 4 to 12 wk after the surgical procedure.Follow-up colonoscopy was performed every 4 wk for 12 wk.The effect of treatment was evaluated using video-endoscopic and histologic scores and measuring interleukin-1β,tumor necrosis factor-alpha,and transforming growth factor beta production in organ cultures by enzyme linked immunosorbent assay.RESULTS:Colonoscopies of the diverted segment showed mucosa with hyperemia,increased number of vessels,bleeding and mucus discharge.Treatment with either glutamine or butyrate induced significant reductions in both colonoscopic (P ＜ 0.02) and histological scores (P ＜ 0.01) and restored the densities of collagen fibers in tissue (P =0.015; P =0.001),the number of goblet cells (P =0.021; P =0.029),and the rate of apoptosis within the epithelium (P =0.043; P =0.011) to normal values.The high levels of cytokines in colon explants from rats with diversion colitis significantly decreased to normal values after treatment with butyrate or glutamine.CONCLUSION:The improvement of experimental diversion colitis following glutamine or butyrate enemas highlights the importance of specific luminal nutrients in the homeostasis of the colonic mucosa and supports their utilization for the treatment of human diversion colitis.

Diversion colitisButyrateGlutamineShortchain fatty acidsCytokines

Rodrigo Goulart Pacheco、Christiano Costa Esposito、Lucas CM Müller、Morgana TL Castelo-Branco、Leonardo Pereira Quintella、Vera Lucia A Chagas、Heitor Siffert P de Souza

展开 >

Department of Surgery,Experimental Surgery Center, Surgical Sciences Postgraduate Program, Medical School, Federal University of Rio de Janeiro,Rio de Janeiro, RJ 21941-913, Brazil

Laboratory of Cellular Immunology, Department of Histology and Embryology, Institute of Biomedical Sciences, Federal University of Rio de Janeiro, Rio de Janeiro, RJ 21941-913, Brazil

Department of Pathology, Medical School, Federal University of Rio de Janeiro, Rio de Janeiro, RJ 21941-913, Brazil

Department of Internal Medicine,Hospital Universitario Clementino Fraga Filho, Federal University of Rio de Janeiro, Rio de Janeiro, RJ 21941-913, Brazil

展开 >

2012

10.3748/wjg.v18.i32.4278

世界胃肠病学杂志(英文版)
太原消化病研治中心

世界胃肠病学杂志(英文版)

SCI
影响因子：1.001
ISSN：1007-9327
年,卷(期)：2012.18(32)
  • 7
  • 50