首页|Development of a quantum-dot-labelled magnetic immunoassay method for circulating colorectal cancer cell detection

Development of a quantum-dot-labelled magnetic immunoassay method for circulating colorectal cancer cell detection

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AIM:To detect of colorectal cancer (CRC) circulating tumour cells (CTCs) surface antigens,we present an assay incorporating cadmium selenide quantum dots (QDs) in these paper.METHODS:The principle of the assay is the immunomagnetic separation of CTCs from body fluids in conjunction with QDs,using specific antibody biomarkers:epithelial cell adhesion molecule antibody,and monoclonal cytokeratin 19 antibody.The detection signal was acquired from the fluorescence signal of QDs.For the evaluation of the performance,the method under study was used to isolate the human colon adenocarcinoma cell line (DLD-1) and CTCs from CRC patients' peripheral blood.RESULTS:The minimum detection limit of the assay was defined to 10 DLD-1 CRC cells/mL as fluorescence was measured with a spectrofluorometer.Fluorescenceactivated cell sorting analysis and Real Time RT-PCR,they both have also been used to evaluate the performance of the described method.In conclusion,we developed a simple,sensitive,efficient and of lower cost (than the existing ones) method for the detection of CRC CTCs in human samples.We have accomplished these results by using magnetic bead isolation and subsequent QD fluorescence detection.CONCLUSION:The method described here can be easily adjusted for any other protein target of either the CTC or the host.

Circulating tumor cellsCancerQuantum dotsNanoprobesMicrometastasis

Maria Gazouli、Anna Lyberopoulou、Pericles Pericleous、Spyros Rizos、Gerassimos Aravantinos、Nikolaos Nikiteas、Nicholas P Anagnou

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Department of Basic Medical Sciences, Laboratory of Biology,School of Medicine, University of Athens, 11527 Athens, Greece

First Department of Surgery,"Tzaneion" General Hospital, 18536 Piraeus, Greece

Third Department of Internal Medicine, Agii Anargyri Hospital, 14564 Athens, Greece

Second Department of Propedeutic Surgery,University of Athens School of Medicine, Laiko General Hospital, 11527 Athens, Greece

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2012

10.3748/wjg.v18.i32.4419

世界胃肠病学杂志(英文版)
太原消化病研治中心

世界胃肠病学杂志(英文版)

SCI
影响因子：1.001
ISSN：1007-9327
年,卷(期)：2012.18(32)
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