首页|Side population cells isolated from KATO Ⅲ human gastric cancer cell line have cancer stem cell-like characteristics

Side population cells isolated from KATO Ⅲ human gastric cancer cell line have cancer stem cell-like characteristics

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AIM:To investigate whether the side population (SP)cells possess cancer stem cell-like characteristics in vitro and the role of SP cells in tumorigenic process in gastric cancer.METHODS:We analyzed the presence of SP cells in different human gastric carcinoma cell lines,and then isolated and identified the SP cells from the KATO Ⅲ human gastric cancer cell line by flow cytometry.The clonogenic ability and self-renewal were evaluated by clone and sphere formation assays.The related genes were determined by reverse transcription polymerase chain reaction.To compare tumorigenic ability,SP and non-side population (NSP) cells from the KATO Ⅲ human gastric cancer cell line were subcutaneously injected into nude mice.RESULTS:SP cells from the total population accounted for 0.57％ in KATO Ⅲ,1.04％ in Hs-746T,and 0.02％ in AGS (CRL-1739).SP cells could grow clonally and have self-renewal capability in conditioned media.The expression of ABCG2,MDRI,Bmi-1 and Oct-4 was different between SP and NSP cells.However,there was no apparent difference between SP and NSP cells when they were injected into nude mice.CONCLUSION:SP cells have some cancer stem celllike characteristics in vitro and can be used for studying the tumorigenic process in gastric cancer.

Side populationCancer stem cellsSelf-renewalGastric cancerKATO Ⅲ

Jun-Jun She、Peng-Ge Zhang、Xuan Wang、Xiang-Ming Che、Zi-Ming Wang

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Department of General Surgery,First Affiliated Hospital of Medical College, Xi'an Jiaotong University, Xi'an 710061, Shaanxi Province, China

Department of Mathematics, Xidian University, Xi'an 710061, Shaanxi Province, China

Department of Endocrinology, First Affiliated Hospital of Medical College, Xi'an Jiaotong University, Xi'an 710061, Shaanxi Province, China

Department of Urology, Second Affiliated Hospital of Medical College, Xi'an Jiaotong University, Xi'an 710004,Shaanxi Province, China

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国家自然科学基金国家自然科学基金China Postdoctoral Science Foundation and NoChina Postdoctoral Science Foundation and NoPhD Programs Fund of the Chinese Ministry of EducationInternational Cooperation Program of Shaanxi ProvinceScience and Technology Program of Shaanxi ProvinceBasic Research Funds for the Central Universities

810721083060061520090450167201003676200902011200682012KW-382010K14-02

2012

10.3748/wjg.v18.i33.4610

世界胃肠病学杂志(英文版)
太原消化病研治中心

世界胃肠病学杂志(英文版)

SCI
影响因子：1.001
ISSN：1007-9327
年,卷(期)：2012.18(33)
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