首页|Decreased mitochondrial deoxyribonucleic acid and increased oxidative damage in chronic hepatitis C
Decreased mitochondrial deoxyribonucleic acid and increased oxidative damage in chronic hepatitis C
扫码查看
点击上方二维码区域，可以放大扫码查看
原文链接
NETL
NSTL
万方数据
维普
AIM:To determine whether alteration of the mitochondria DNA (mtDNA) copy number and its oxidative damage index (mtDNA△CT) can be detected by analysis of peripheral blood cells in hepatitis C virus (HCV)-infected patients.METHODS:This study enrolled two groups of patients aged 40-60 years:a control group and an HCV-infected group in Department of Gastroenterology and Hepatology in Changhua Christian Hospital.Patients with co-infection with hepatitis B virus or human immunodeficiency virus,autoimmune disease,malignant neoplasia,pregnancy,thyroid disease,or alcohol consumption ＞ 40 g/d were excluded.HCV-infected patients who met the following criteria were included:(1)positive HCV antibodies for ＞ 6 mo; (2) alanine aminotransferase (ALT) levels more than twice the upper limit of normal on at least two occasions during the past 6 mo; and (3) histological fibrosis stage higher than F1.The mtDNA copy number and oxidative damage index of HCV mtDNA (mtDNA△CT) were measured in peripheral blood leukocytes.The association between mtDNA copy number and mtDNA△Cr was further analyzed using clinical data.RESULTS:Forty-seven normal controls (male/female:26/21,mean age 50.51 ± 6.15 years) and 132 HCV-infected patients (male/female:76/61,mean age 51.65± 5.50 years) were included in the study.The genotypes of HCV-infected patients include type 1a (n =3),type 1b (n =83),type 2a (n =32),and type 2b (n =14).Liver fibrosis stages were distributed as follows:F1/F2/F3/F4 =1/61/45/25 and activity scores were A0/A1/A2/A3 =7/45/55/25.There were no age or genderdifferences between the two groups.HCV-infected patients had higher hepatitis activity (aspartate transaminase levels 108.77 ± 60.73 vs 23.19 ± 5.47,P ＜ 0.01;ALT levels 168.69 ± 93.12 vs 23.15 ± 9.45,P ＜ 0.01)and lower platelet count (170.40 ± 58.00 vs 251.24 ±63.42,P ＜ 0.01) than controls.The mtDNA copy number was lower in HCV-infected patients than in controls (173.49 vs 247.93,P ＜ 0.05).The mtDNA△CT was higher in HCV-infected patients than in controls (2.92 vs 0.64,P ＜ 0.05).To clarify the clinical significance of these results in HCV-infected patients,their association with different clinical parameters among HCV-infected patients was analyzed.A negative association was found between mtDNA copy number and elevated aspartate transaminase levels (r =-0.17,P ＜ 0.05).Changes in mtDNA copy number were not associated with HCV RNA levels,HCV genotypes,liver fibrosis severity,or inflammatory activity in the liver biopsy specimen.However,a correlation was observed between mtDNA△Cr and platelet count (r =-0.22,P ＜ 0.01),HCV RNA level (r =0.36,P ＜ 0.01),and hepatitis activity (r =0.20,P =0.02).However,no difference in the change in mtDNA△Crwas observed between different fibrosis stages or HCV CONCLUSION:Oxidative stress and mtDNA damage are detectable in patient's peripheral leukocytes.Increased leukocyte mtDNA△CT correlates with higher HCV viremia,increased hepatitis activity,and lower platelet count.
NETL
NSTL
万方数据
维普
