首页|Melatonin attenuates acute pancreatitis-associated lung injury in rats by modulating interleukin 22

Melatonin attenuates acute pancreatitis-associated lung injury in rats by modulating interleukin 22

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AIM:To investigate whether therapeutic treatment with melatonin could protect rats against acute pancreatitis and its associated lung injury.METHODS:Seventy-two male Sprague-Dawley rats were randomly divided into three groups:the sham operation (SO),severe acute pancreatitis (SAP),and melatonin treatment (MT) groups.Acute pancreatitis was induced by infusion of 1 mL/kg of sodium taurocholate (4％ solution) into the biliopancreatic duct.Melatonin (50 mg/kg) was administered 30 min before pancreatitis was induced,and the severity of pancreatic and pulmonary injuries was evaluated 1,4 and 8 h after induction.Serum samples were collected to measure amylase activities,and lung tissues were removed to measure levels of mRNAs encoding interleukin 22 (IL-22)and T helper cell 22 (Th22),as well as levels of IL-22.RESULTS:At each time point,levels of mRNAs encoding IL-22 and Th22 were significantly higher (P ＜ 0.001)in the MT group than in the SAP group (0.526 ± 0.143vs 0.156 ± 0.027,respectively,here and throughout,after 1 h; 0.489 ± 0.150 vs 0.113 ±0.014 after 4 h;0.524 ± 0.168 vs 0.069 ± 0.013 after 8 h,0.378 ± 0.134vs 0.122 ± 0.015 after 1 h; 0.205 ± 0.041 vs 0.076 ±0.019 after 4 h; 0.302 ± 0.108 vs 0.045 ± 0.013 after 8 h,respectively) and significantly lower (P ＜ 0.001)in the SAP group than in the SO group (0.156 ± 0.027vs 1.000 ± 0.010 after 1 h; 0.113 ± 0.014 vs 1.041 ±0.235 after 4 h; 0.069 ± 0.013 vs 1.110 ± 0.213 after 8 h,0.122 ± 0.015 vs 1.000 ± 0.188 after 1 h; 0.076 ± 0.019 vs 0.899 ± 0.125 after 4 h; 0.045 ± 0.013 vs 0.991± 0.222 after 8 h,respectively).The mean pathological scores for pancreatic tissues in the MT group were significantly higher (P ＜ 0.01) than those for samples in the SO group (1.088 ± 0.187 vs 0.488 ± 0.183 after 1 h;2.450 ± 0.212 vs 0.469 ± 0.242 alter 4 h; 4.994 ± 0.184vs 0.513 ± 0.210 alter 8 h),but were significantly lower (P ＜ 0.01) than those for samples in the SAP group at each time point (1.088 ± 0.187 vs 1.969 ± 0.290 after 1 h; 2.450 ± 0.212 vs 3.344 ± 0.386 after 4 h; 4.994 ±0.184 vs 6.981 ± 0.301 after 8 h).The severity of SAP increased significantly (P ＜ 0.01) over time in the SAP group (1.088 ± 0.187 vs 2.450 ± 0.212 between 1 hand 4 h after inducing pancreatitis; and 2.450 ± 0.212vs 4.994 ± 0.184 between 4 and 8 h after inducing pancreatitis).CONCLUSION:Melatonin protects rats against acute pancreatitis-associated lung injury,probably through the upregulation of IL-22 and Th22,which increases the innate immunity of tissue cells and enhances their regeneration.

Interleukin 22MelatoninPancreatitisassociated lung injurySevere acute pancreatitisT helper 22 cell

Jia-Ping Huai、Xue-Cheng Sun、Meng-Jun Chen、Yin Jin、Xiao-Hua Ye、Jian-Sheng Wu、Zhi-Ming Huang

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Department of Gastroenterology, First Affiliated Hospital of Wenzhou Medical College, Wenzhou 325000, Zhejiang Province, China

Wenzhou Municipal Science and Technology Commission major projects fundsscientific committee of Wenzhou and the Department of Gastroenterology and Hepatology,First Affiliated Hospital,Wenzhou Medi

20090006

2012

10.3748/wjg.v18.i36.5122

世界胃肠病学杂志(英文版)
太原消化病研治中心

世界胃肠病学杂志(英文版)

SCI
影响因子：1.001
ISSN：1007-9327
年,卷(期)：2012.18(36)
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