首页|CpG island methylator phenotype and Helicobacter pylori infection associated with gastric cancer
CpG island methylator phenotype and Helicobacter pylori infection associated with gastric cancer
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AIM:TO investigate the association between the CpG island methylator phenotype (CIMP) and serum Helicobacter pylori (H.pylori) levels for clinical prediction of gastric cancer (GC) progression.METHODS:We analyzed the serum CIMP status of 75 patients with GC using a methylation marker panel and a methylation-specific polymerase chain reaction.Serum samples from 40 healthy persons were examined at the same time.The genes examined were APC,WIF-1,RUNX-3,DLC-1,SFRP-1,DKK and E-cad.H.pylori infection in serum was assayed with an anti-H.pylori immunoglobulin G antibody test and a rapid urease test.RESULTS:The frequencies of high-level methylation in GC tissues for the seven genes were:48％ for APC,57.33％ for WIF-1,56％ for RUNX-3,50.67％ for DLC-1,52％ for SFRP-1,54.67％ for DKK,and 48％ for E-cad.The frequencies in GC serum were 30.67％ for APC,34.67％ for WIF-1,37.33％ for RUNX-3,29.33％ for DLC-1,33.33％ for SFRP-1,32％ for DKK,and 26.67％for E-cad.CIMP+ (defined as ≥ 3 methylated genes)was associated with 47 (62.67％) GC tissue samples and 44 (58.67％) GC serum samples.CIMP+ was not associated with non-neoplastic mucosal tissues or the serum of healthy persons.Of the 75 GC cases,51 (68％)were H.pylori+,and 24 (32％) were H.pylori-.Of the 51 H.pylori+ cases,36 were CIMP+ and 15 were CIMP-.In contrast,for the 24H.pylori-cases,11 were ClMP+,and 13 were CIMP-.The difference was significant between the H.pylori+ and H.pylori-groups (x2=4.27,P ＜ 0.05).Of the 51 H.pylori+ GC patients,34were CIMP+ and 17 were CIMP-,while among the 24 H.pylori-GC cases,10 were CIMP+ and 14 were CIMP-.The difference was significant between the H.pylori+and H.pylori-groups (x2 =4.21,P ＜ 0.05).A 2-year follow-up showed significant difference in the rates of metastasis and recurrence between H.pylori+lCIMP+cases and the H.pylori+/CIMP-cases or CIMP-cases associated with H.pylori assayed in serum (P ＜ 0.05).However,there were no significant differences in survival rates between the two groups.CONCLUSION:H.pylori+/CIMP+ cases are associated with higher rates of metastasis and recurrence than H.pylori+/CIMP-cases.Serum may be useful for examining CIMP status.
CpG island methylator phenotypeHelicobacter pyloriSerumPrognosisGastric cancer
Ji-Bin Liu、Xu-Ming Wu、Jin Cai、Jin-Ye Zhang、Jin-Lin Zhang、Shu-Hui Zhou、Min-Xin Shi
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Tumor Institute, Nantong Tumor Hospital, Nantong 226361, Jiangsu Province, China
School of Radiology and Public Health, Soochow University, Suzhou 215123, Jiangsu Province, China
NETL
NSTL
万方数据
