首页|Nur-related receptor 1 gene polymorphisms and alcohol dependence in Mexican Americans

Nur-related receptor 1 gene polymorphisms and alcohol dependence in Mexican Americans

扫码查看
原文链接
  • NETL
  • NSTL
  • 万方数据
  • 维普
AIM:To investigate the association of polymorphisms of nut-related receptor 1 (Nurr1) and development of alcohol dependence in Mexican Americans.METHODS:Peripheral blood samples were collected from 374 alcoholic and 346 nonalcoholic Mexican Americans; these two groups were sex-and age-matched.Sample DNA was extracted and genomic DNA was amplified by polymerase chain reaction.The-2922(C) 2-3 polymerase chain reaction products were digested with Sau96I,alleles of 1345(G/C),and-1198(C/G) in the regulatory region as well as Ex+132 (G/T/A/C) and Ex+715(T/-) in exon 3 were studied by sequencing.RESULTS:The C2/C2,C2/C3,C3/C3 genotype distribution of-2922(C) 2-3 was 34.4％,38.2％ and 27.5％ in the nonalcoholic group compared to 23.3％,51.2％ and 25.4％ in the alcoholic group (P =0.001).The C/C,C/G,G/G genotype distribution of-1198(C/G) was 23.5％,46.1％ and 30.3％ in the nonalcoholic group compared to 13.9％,50.9％ and 35.3％ in the alcoholic group (P =0.007).However,the-1345 (G/C),Ex3+132(G/T/A/C) and Ex3+715(T/-) alleles were not polymorphic in Mexican Americans,and all those studied had G/G,G/G and T/T genotype for these three alleles,respectively.The -2922(C) 2-3 did not show allele level difference between alcoholic and nonalcoholic individuals,but-1198 (C/G) showed a significant allele frequency difference between alcoholic (39.3％) and nonalcoholic (46.6％) populations (P =0.005).Excluding obese individuals,significant differences were found at both genotypic and allelic levels for the-2922(C) 2-3 polymorphism (P =0.000 and P =0.049) and the-1198 (C/G) polymorphism (P =0.008 and P =0.032) between nonobese alcoholics and nonobese controls.Excluding smokers,a significant difference was found only at the genotypic level for the-2922(C) 2-3 polymorphism (P =0.037) between nonsmoking alcoholics and nonsmoking controis,but only at the allelic level for the-1198(C/G) polymorphism (P =0.034).CONCLUSION:Polymorphisms in the regulatory region of Nurr1 are implicated in pathogenesis of alcohol dependence and the Nurr1/dopamine signaling pathway might be important for this dependence development in Mexican Americans.

Nur-related receptor 1PolymorphismAlcohol dependenceObesitySmokingNuclear receptor

Ya-Ming Wei、Yan-Lei Du、Yu-Qiang Nie、Yu-Yuan Li、Yu-Jui Yvonne Wan

展开 >

Department of Blood Transfusion, Guangzhou First People's Hospital, Guangzhou Medical University, Guangzhou 510180, Guangdong Province, China

Department of Gastroenterology and Hepatology, Guangzhou First People's Hospital, Guangzhou Medical University, Guangzhou 510180, Guangdong Province, China

Department of Pharmacology, Toxicology and Therapeutics, The University of Kansas Medical Center,Kansas City, KS 66160, United States

NIH/NIAAA Grant RO1 AA 12081Centers of Biomedical Research Excellence

Grant P20 RR021940

2012

10.3748/wjg.v18.i37.5276

世界胃肠病学杂志(英文版)
太原消化病研治中心

世界胃肠病学杂志(英文版)

SCI
影响因子：1.001
ISSN：1007-9327
年,卷(期)：2012.18(37)
  • 1
  • 38