首页|Myofibrillogenesis regulator-1 overexpression is associated with poor prognosis of gastric cancer patients

Myofibrillogenesis regulator-1 overexpression is associated with poor prognosis of gastric cancer patients

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AIM:To investigate the expression of myofibrillogenesis regulator-1 (MR-1) in relation to clinicopathological parameters and postoperative survival in a group of Chinese patients with gastric cancer.METHODS:In our previous study of human wholegenome gene expression profiling,the differentially expressed genes were detected in the gastric cancer and its adjacent noncancerous mucosa.We found that MR-1 was associated with the location and differentiation of tumors.In this study,MR-1 protein expression was determined by immunohistochemistry in specimens of primary cancer and the adjacent noncancerous tissues from gastric cancer patients.A set of real-time quantitative polymerase chain reaction assays based on the Universal ProbeLibrary-a collection of 165 presynthesized,fluorescence-labeled locked nucleic acid hydrolysis probes-was designed specifically to detect the expression of MR-1 mRNA.The correlation was analyzed between the expression of MR-1 and other tumor characteristics which may influence the prognosis of gastric cancer patients.A retrospective cohort study on the prognosis was carried out and clinical data were collected from medical records.RESULTS:MR-1 mRNA and protein could be detected in gastric cancer tissues as well as in matched noncancerous tissues.MR-1 was up-regulated at both mRNA (5.459 ± 0.639 vs 1.233 ± 0.238,P ＜ 0.001) and protein levels (34.2％ vs 13.2％,P =0.003) in gastric cancer tissues.Correlation analysis demonstrated that high expression of MR-1 in gastric cancer was significantly correlated with clinical stage (P =0.034).Kaplan-Meier analysis showed that the postoperative survival of the MR-1 positive group tended to be poorer than that of the MR-1 negative group,and the difference was statistically significant (P =0.002).Among all the patients with stage Ⅰ-Ⅳ carcinoma,the 5-year survival rates of MR-1 positive and negative groups were 50.40％ and 12.70％,respectively,with respective median survival times of 64.27 mo (95％CI:13.41-115.13) and 16.77mo (95％CI:8.80-24.74).Univariate and multivariate analyses were performed to compare the impact of MR-1 expression and other clinicopathological parameters on prognosis.In a univariate analysis on all 70specimens,6 factors were found to be significantly associated with the overall survival statistically:including MR-1 expression,depth of invasion,distant metastasis,lymph node metastasis,vascular invasion and the tumor node metastasis (TNM) stage based on the 7th edition of the International Union against Cancer TNM classification.To avoid the influence caused by univariate analysis,the expressions of MR-1 as well as other parameters were examined in multivariate Cox analysis.Clinicopathological variables that might affect the prognosis of gastric cancer patients were analyzed by Cox regression analysis,which showed that MR-1 expression and TNM stage were independent predictors of postoperative survival.The best mathematical multivariate Cox regression model consisted of two factors:MR-1 expression and TNM stage.Our results indicated that MR-1 protein could act as an independent marker for patient overall survival [Hazard ratio (HR):2.215,P =0.043].CONCLUSION:4R-1 is an important variable that can be used to evaluate the outcome,prognosis and targeted therapy of gastric cancer patients.

Myofibrillogenesis regulator-1Gastric cancerReal-time quantitative reverse transcriptase-polymerase chain reactionImmunohistochemistryPoor prognosis

Jing Guo、Bin Dong、Jia-Fu Ji、Ai-Wen Wu

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Key Laboratory of Carcinogenesis and Translational Research(Ministry of Education),Department of Gastrointestinal Surgery, Peking University Cancer Hospital, Beijing Cancer Hospital and Institute, Beijing 100142, China

Key Laboratory of Carcinogenesis and Translational Research(Ministry of Education),Department of Pathology,Peking University Cancer Hospital, Beijing Cancer Hospital and Institute, Beijing 100142, China

National 863 ProgramNational 863 ProgramBeijing Municipal Committee of Science and TechnologyBeijing Capital Development Foundation of Health Bureau

SQ2009AA02-XK14825702006AA02A402D09050010406312007-2051

2012

10.3748/wjg.v18.i38.5434

世界胃肠病学杂志(英文版)
太原消化病研治中心

世界胃肠病学杂志(英文版)

SCI
影响因子：1.001
ISSN：1007-9327
年,卷(期)：2012.18(38)
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