首页|Effect of the ginsenoside Rb1 on the spontaneous contraction of intestinal smooth muscle in mice

Effect of the ginsenoside Rb1 on the spontaneous contraction of intestinal smooth muscle in mice

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AIM:To investigate the effect and the possible mechanism of ginsenoside Rb1 on small intestinal smooth muscle motility in mice.METHODS:Intestinal smooth muscle strips were isolated from male ICR mice (5 wk old),and the effect of ginsenoside Rb1 on spontaneous contraction was recorded with an electrophysiolograph.The effect of ginsenoside Rb1 on ion channel currents,including the voltage-gated K+ channel current (IKv),calcium-activated potassium channel currents (IKCa),spontaneous transient outward currents and ATP-sensitive potassium channel current (IKATP),was recorded on freshly isolated single cells using the whole-cell patch clamp technique.RESULTS:Ginsenoside Rb1 dose-dependently inhibited the spontaneous contraction of intestinal smooth muscle by 21.15％ ± 3.31％,42.03％ ± 8.23％ and 67.23％ ± 5.63％ at concentrations of 25 μmol/L,50μmol/L and 100 μmol/L,respectively (n =5,P ＜ 0.05).The inhibitory effect of ginsenoside Rb1 on spontaneous contraction was significantly but incompletely blocked by 10 mmol/L tetraethylammonium or 0.5mmol/L 4-aminopyridine,respectively (n =5,P ＜ 0.05).However,the inhibitory effect of ginsenoside Rb1 on spontaneous contraction was not affected by 10 μmol/Lglibenclamide or 0.4 μmol/L tetrodotoxin.At the cell level,ginsenoside Rb1 increased outward potassium currents,and Ikv was enhanced from 1137.71 ± 171.62pA to 1449.73 ± 162.39 pA by 50 μmol/L Rb1 at +60mV (n =6,P ＜ 0.05).Ginsenoside Rb1 increased IKCa and enhanced the amplitudes of spontaneous transient outward currents from 582.77 ± 179.09 mV to 788.12± 278.34 mV (n =5,P ＜ 0.05).However,ginsenoside Rb1 (50 μmol/L) had no significant effect on IKATP (n =3,P ＜ 0.05).CONCLUSION:These results suggest that ginsenoside Rb1 has an inhibitory effect on the spontaneous contraction of mouse intestinal smooth muscle mediated by the activation of Ikv and IKCa,but the KATP channel was not involved in this effect.

Ginsenoside Rb1Intestinal smooth muscleIntestinal smooth muscle cellPotassium channelSpontaneous contractionWhole-cell patch clamp technique

Lei Xu、Sui-Ping Huang

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Scientific Research Station for Post-doctoral Studies,Post-doctoral Research Centre, Zhongshan Affiliated Hospital,Guangzhou University of Traditional Chinese Medicine, Zhongshan 528400, Guangdong Province, China

Department of Gastroenterology, Guangdong Provincial Traditional Chinese Medicine Hospital, Ersha Island Branch, Guangzhou 510405, Guangdong Province, China

国家自然科学基金State Administration of Traditional Chinese Medicine of the People's Republic of China

3087332806-075930

2012

10.3748/wjg.v18.i38.5462

世界胃肠病学杂志(英文版)
太原消化病研治中心

世界胃肠病学杂志(英文版)

SCI
影响因子：1.001
ISSN：1007-9327
年,卷(期)：2012.18(38)
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