首页|Thioredoxin and thioredoxin-interacting protein as prognostic markers for gastric cancer recurrence

Thioredoxin and thioredoxin-interacting protein as prognostic markers for gastric cancer recurrence

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To evaluate the potential of thioredoxin (TXN)and thioredoxin-interacting protein (TXNIP) expression as biomarkers for predicting gastric cancer recurrence.METHODS:TXN and TXNIP expression levels were acquired from gene expression microarray data for 65human gastric cancer tissues.We determined whether each gene expression level was associated with cancer recurrence and investigated the relationship between the two genes.For validation,the expression levels of TXN and TXNIP were measured by quantitative realtime reverse transcription polymerase chain reaction in 68 independent stage Ⅲ gastric cancer patients.The correlation between gene expression and cancer prognosis was evaluated.Immunohistochemical staining was performed to investigate the protein expression levels of TXN and TXNIP and to characterize the expression patterns of each protein.RESULTS:TXN was a prognosis-related gene (P =0.009),whereas TXNIP,a TXN inhibitor,demonstrated a negative correlation with TXN in the gene expression microarray data.In the 68 stage Ⅲ patients,the expression levels of both TXN and TXNIP had a statistically significant effect on recurrence-free survival (RFS,P =0.008 and P =0.036,respectively).The low TXN and high TXNIP expression group exhibited a better prognosis than the other groups,and the high TXN and low TXNIP expression group exhibited a poorer prognosis (P ＜ 0.001 for RFS and P =0.001 for overall survival).More than half of the patients in the simultaneously high TXN and low TXNIP expression group experienced a recurrence within 1 year after curative surgery,and the 5-year survival rate of the patients in this group was 29％,compared with 89％ in the low TXN and high TXNIP expression group.The TXN protein was overexpressed in 65％ of the gastric cancer tissues,whereas the TXNIP protein was underexpressed in 85％ of the cancer cells.In a correlation analysis,TXN and TXNIP were highly correlated with many oncogenes and tumor suppressors as well as with genes related to energy,protein synthesis and autophagy.CONCLUSION:TXN and TXNIP are promising prognostic markers for gastric cancer,and performing personalized adjuvant treatment based on TXN and TXNIP expression levels would be an effective practice in the treatment of gastric cancer.

Gastric cancerThioredoxinThioredoxininteracting proteinBiomarkerPrognosis

Jae Yun Lim、Sun Och Yoon、Soon Won Hong、Jong Won Kim、Seung Ho Choi、Jae Yong Cho

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Department of Medical Oncology, Gangnam Severance Cancer Hospital, Yonsei University College of Medicine, Seoul 135-720, South Korea

Department of Pathology,Gangnam Severance Cancer Hospital, Yonsei University College of Medicine, Seoul 135-720, South Korea

Department of Surgery,Gangnam Severance Cancer Hospital, Yonsei University College of Medicine, Seoul 135-720, South Korea

Basic Science Research Program through the National Research Foundation of Korea funded by the Ministry of Education,ScienceA Faculty Research Grant from Yonsei University College of Medicine for 2011A Faculty Research Grant from Yonsei University College of Medicine for 2011

2010-00242486-2011-01136-2011-0146

2012

10.3748/wjg.v18.i39.5581

世界胃肠病学杂志(英文版)
太原消化病研治中心

世界胃肠病学杂志(英文版)

SCI
影响因子：1.001
ISSN：1007-9327
年,卷(期)：2012.18(39)
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