首页|Correlation between mitochondrial TRAP-1 expression and lymph node metastasis in colorectal cancer
Correlation between mitochondrial TRAP-1 expression and lymph node metastasis in colorectal cancer
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NETL
NSTL
万方数据
维普
AIM:To evaluate the effect of mitochondrial tumor necrosis factor receptor-associated protein-1 (TRAP-1) on the lymph node metastasis (LNM) in Chinese colorectal cancer (CRC) patients,and develop potential LNM-associated biomarkers for CRC using quantitative realtime polymerase chain reaction (RT-PCR) analysis.METHODS:Differences in mitochondrial TRAP-1 gene expression between primary CRC with LNM (LNM CRC) and without LNM (non-LNM CRC) were assessed in 96 Chinese colorectal carcinoma samples using quantitative RT-PCR analysis,Western blotting,and confirmed with immunohistochemical assay.The relationship between clinicopathological parameters and potential diagnostic biomarkers was also examined.RESULTS:TRAP-1 was significantly upregulated in LNM CRC compared with non-LNM CRC,which was confirmed by RT-PCR,Western blotting and immunohistochemical assay.The expression of TRAP-1 in two different metastatic potential human colorectal cancer cell lines,LoVo and HT29,was analyzed with Western blotting.The expression level of TRAP-1 was dramatically higher in LoVo than in HT29.Overexpression of TRAP-1 was significantly associated with LNM (90.2％ in LNM group vs 22％ in non-LNM group,P ＜ 0.001),the advanced tumor node metastasis stage (89.1％ in LNM group vs 26.9％ in non-LNM group,P ＜ 0.001),the increased 5-year recurrence rate (82.7％ in LNM group vs 22.6％ in non-LNM group,P ＜ 0.001) and the decreased 5-year overall survival rate (48.4％ in LNM vs 83.2％ in non-LNM group,P ＜ 0.001).Univariate and multivariate analyses indicated that TRAP-1 expression was an independent prognostic factor for recurrence and survival of CRC patients (Hazard ratio of 2.445 in recurrence,P =0.017; 2.867 in survival,P =0.028).CONCLUSION:Mitochondria TRAP-1 affects the lymph node metastasis in CRC,and may be a potential biomarker for LNM and a prognostic factor in CRC.Over-expression of TRAP-1 is a predictive factor for the poor outcome of colorectal cancer patients.
NETL
NSTL
万方数据
维普
