首页|Magnetic resonance-based total liver volume and magnetic resonance-diffusion weighted imaging for staging liver fibrosis in mini-pigs

Magnetic resonance-based total liver volume and magnetic resonance-diffusion weighted imaging for staging liver fibrosis in mini-pigs

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AIM:TO determine whether and how magnetic resonance imaging (MRI)-based total liver volume (TLV) and diffusion weighted imaging (DWI) could predict liver fibrosis.METHODS:Sixteen experimental mature mini-pigs (6 males,10 females),weighing between 20.0 and 24.0 kg were prospectively used to model liver fibrosis induced by intraperitoneal injection of 40％ CCl4 dissolved in fat emulsion twice a week for 16 wk,and by feeding 40％ CCl4 mixed with maize flour twice daily for the subsequent 5 wk.All the survival animals underwent percutaneous liver biopsy and DWI using b =300,500 and 800 s/mm2 followed by abdominal gadolinium-enhanced MRI at the 0,5th,9th,16th and 21st weekend after beginning of the modeling.TLV was obtained on enhanced MRI,and apparent diffusion coefficient (ADC) was obtained on DWI.Hepatic tissue specimens were stained with hematoxylin and Masson's trichrome staining for staging liver fibrosis.Pathological specimens were scored using the human METAVIR classification system.Statistical analyses were performed to determine whether and how the TLV and ADC could be used to predict the stage of liver fibrosis.RESULTS:TLV increased from stage 0 to 2 and decreased from stage 3 (r =0.211; P ＜ 0.001).There was a difference in TLV between stage 0-1 and 2-4 (P =0.03) whereas no difference between stage 0-2 and 3-4 (P =0.71).TLV could predict stage ≥ 2 [area under receiver operating characteristic curve (AUC) =0.682].There was a decrease in ADC values with increasing stage of fibrosis for b =300,500 and 800s/mm2 (r =-0.418,-0.535 and-0.622,respectively;all P ＜ 0.001).Differences were found between stage 0-1 and 2-4 in ADC values for b =300,500 and 800 s/mm2,and between stage 0-2 and 3-4 for b =500 or 800 s/mm2 (all P ＜ 0.05).For predicting stage ≥ 2 and ≥ 3,AUC was 0.803 and 0.847 for b =500 s/mm2,and 0.848 and 0.887 for b =800 s/mm2,respectively.CONCLUSION:ADC for b =500 or 800 s/mm2 could be better than TLV and ADC for b =300 s/mm2 to predict fibrosis stage ≥ 2 or ≥ 3.

Magnetic resonance imagingTotal liver volumeLiver fibrosisApparent diffusion coefficientStage

Hang Li、Tian-Wu Chen、Xiao-Li Chen、Xiao-Ming Zhang、Zhen-Lin Li、Nan-Lin Zeng、Li Zhou

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Sichuan Key Laboratory of Medical Imaging,Department of Radiology, Affiliated Hospital of North Sichuan Medical College, Nanchong 637000, Sichuan Province, China

Department of Radiology, West China Hospital of Sichuan University, Chengdu 610041, Sichuan Province, China

国家自然科学基金Key Projects in the Sichuan Province Science and Technology Pillar ProgramScience Fund for Distinguished Young Scholars of Sichuan Province,China

810500332011SZ02372010JQ0039

2012

10.3748/wjg.v18.i48.7225

世界胃肠病学杂志(英文版)
太原消化病研治中心

世界胃肠病学杂志(英文版)

SCI
影响因子：1.001
ISSN：1007-9327
年,卷(期)：2012.18(48)
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