首页|Single nucleotide polymorphisms in the CDH17 gene of colorectal carcinoma

Single nucleotide polymorphisms in the CDH17 gene of colorectal carcinoma

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AIM:To investigate the relationship between c.343A＞G and c.2216A＞C polymorphism sites in the CDH17 gene and colorectal carcinoma.METHODS:Ninety-three non-consanguineous colorectal carcinoma patients admitted to the Department of Oncology at the First Affiliated Hospital of Zhengzhou University were included in this study.Ninety-three peripheral venous blood samples,of approximately one milliliter from each patient,were collected between December 2009 and August 2010.The genomic DNA of these peripheral venous blood samples were extTacted and purified using a Fermentas Genomic DNA Purification Kit (Fermentas,CA) according to the manufacturer's protocol.The single nucleotide polymorphisms (SNPs) of the liver-intestine cadherin (CDH17) gene c.343A＞G and c.2216A＞C were determined by the polymerase chain reaction-single strand conformation polymorphism method (PCR-SSCP) in 93 peripheral venous blood sampies from patients suffering with colorectal carcinoma.Typical samples that showed different migration bands in SSCP were confirmed by sequencing.Directed DNA sequencing was used to check the correctness of the genotype results from the PCR-SSCP method.RESULTS:There was a significant association between the c.2216 A＞C SNPs of the CDH17 gene and the tumor-node-metastasis (TNM) grade,as well as with lymph node status,in 93 peripheral venous blood sampies from colorectal carcinoma patients.The genotype frequencies of A/C,A/A,and C/C were 12.90％,33.33％ and 53.76％,respectively.There was a significant correlation between lymph node metastasis,TNM grade,and the genotype distribution (P ＜ 0.05).The C/C genotype raised the risk of lymph node metastasis and the TNM grade.There was a significant difference in the TNM grade and lymph node metastasis between the A/A and C/C genotypes (P =0.003 and P =0.013,respectively).Patients with colorectal carcinoma carrying the C allele tended to have a higher risk of lymph node metastasis and have a higher TNM grade.The difference between the TNM grades,as well as the lymph node metastasis of the two alleles,was statistically significant (P ＜ 0.01).CONCLUSION:The SNPs of the CDH17 gene c.2216 A＞C might be clinically important in the prognosis of colorectal carcinoma.

Single nucleotide polymorphismsLiverintestine cadherinColorectal carcinomaPolymerase chain reaction-single strand conformation polymorphism method

Ren-Yin Chen、Juan-Juan Cao、Juan Chen、Jian-Ping Yang、Xiao-Bo Liu、Guo-Qiang Zhao、Yu-Feng Zhang

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Department of Pathology, the First Affiliated Hospital of Zhengzhou University, Zhengzhou 450052, Henan Province, China

Henan Key Laboratory for Tumor Pathology, Zhengzhou 450052, Henan Province, China

Zhengzhou University School of Pharmaceutical Sciences, Zhengzhou 450052, Henan Province, China

Basic Medical College of Zhengzhou University, Zhengzhou 450052, Henan Province, China

Department of Surgery, the First Affiliated Hospital of Zhengzhou University, Zhengzhou 450052, Henan Province, China

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Henan Provincial Department of Education on Natural Science Research Projects

2010A310011

2012

10.3748/wjg.v18.i48.7251

世界胃肠病学杂志(英文版)
太原消化病研治中心

世界胃肠病学杂志(英文版)

SCI
影响因子：1.001
ISSN：1007-9327
年,卷(期)：2012.18(48)
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