Promotion of GDF11 on osteoblast differentiation of ADSCs derived from diabetic mice by activating PI3K/Akt signaling pathway
Objective To investigate the effects of growth differentiation factor 11(GDF11)on osteoblast differentiation of adipose derived stem cells(ADSCs)from diabetic mice and related signaling mechanism.Methods ADSCs from diabetic mice were i-solated and cultured.The effect of GDF11 on the proliferation of ADSCs was detected by flow cytometry.After cultured in osteogenic differentiation medium,ALP activity was detected,qPCR was used to evaluate the expression of osteoblast genes,and phosphorylation levels of PI3K and Akt were detected by western blot.Results Cell proliferation was less affected by GDF11 intervation,promoted ALP activity in a concentration-dependent but saturated manner,significantly upregulated mRNA expression of Runx2(2.41±0.19)vs.(1.00±0.11)],Osx[(1.41±0.21)vs.(1.00±0.10)]and ALP[(1.42±0.20)vs.(1.00±0.09)](P<0.05),and signifi-cantly increased phosphorylation levels of PI3K[(2.84±0.19)vs.(1.00±0.11)]and Akt[(4.58±0.20)vs.(1.00±0.19)](P<0.05).Moreover,the effects of GDF11 on promoting osteoblast differentiation of ADSCs from diabetic mice was partially weak-ened by the PI3K inhibitor LY294002.Conclusions GDF11 promotes osteoblast differentiation of ADSCs from diabetic mice,and its mechanism may be related with the activation of PI3K/Akt signaling pathway.
国家科技期刊平台
NSTL
万方数据
维普
