Value of cuproptosis gene LIPT1 in diagnosis and prognosis of brain gliomas
Objective To investigate the potential value of the cuproptosis gene LIPT1 in the diagnosis and prognosis of glio-ma.Methods The expression of 10 cuproptosis genes in glioma was mined using TCGA database.The expression of LIPT1 in 18 glio-ma patients was detected by IHC and qRT-PCR.The expression of LIPT1 in HEB,U87 and U251 cells was detected by qRT-PCR.ROC curve was used to predict the diagnostic efficacy of LIPT1 in glioma.Kaplan-Meier survival curve and Cox analysis were used to explore the prognostic value of LIPT1.ssGSEA database was used to evaluate the effect of LIPT1 on immune invasion of glioma.To ex-plore the relationship between LIPT1 and immunological checkpoints,DNA methylation site and DNA mismatch repair genes in glio-mas,fFunctional enrichment of LIPT1-associated genes was performed with GO,KEGG,and GSEA.Animal TFDB3 predicts LIPT1-associated transcription factors.The GSCA database was utilized to predict potential drugs targeting LIPT1 and hub genes.Results LIPT1 expression in glioma was higher than in normal tissues(3.03 vs.2.28,P<0.05).The immune scores of grade Ⅱ,Ⅲ and Ⅳ gliomas were different[(1.47±0.51)vs.(4.94±1.25)vs.(9.88±1.65),P<0.05].The mRNA levels of LIPT1 in HEB,U87 and U251 cells were different[(0.22±0.03)vs.(0.45±0.04)vs.(0.58±0.05),P<0.05].The AUC value of LIPT1 in glioma diagnosis was 0.87.The OS,DSS,and PFI in the high LIPT1 expression group were shorter than those in the low LIPT1 expression group(P<0.05).The LIPT1 expression was correlated with immune infiltration changes and the expression of immune check-point,DNA methylation site and DNA mismatch repair gene(P<0.05).Transcription factors such as FOXG1 were predicted to possi-bly bind to LIPT1.Drugs such as docetaxel might treat gliomas by tar-geting and regulating LIPT1-related hub genes.Conclusions LIPT1 may be a potential biomarker and may be associated with poor prog-nosis of glioma.
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