爱帕琳肽13对GK大鼠BMSCs骨向分化及线粒体呼吸功能的影响
Effect of Apelin-13 on osteoblast differentiation and mitochondrial respiration function of BMSCs derived from GK diabetic rats
李梦 1李爽 2张欣然 3朱彪 2郑雪 2王坚1
作者信息
- 1. 100101 北京,战略支援部队特色医学中心口腔科
- 2. 100038 北京,首都医科大学附属复兴医院口腔科
- 3. 100053 北京,首都医科大学宣武医院口腔科
- 折叠
摘要
目的 探讨爱帕琳肽 13(Apelin-13)对GK大鼠BMSCs骨向分化及线粒体呼吸功能的影响.方法 分离、培养GK糖尿病大鼠BMSCs.成骨诱导培养后,检测碱性磷酸酶(ALP)活性,Von Kossa染色观察钙结节矿化程度,O2K线粒体呼吸仪测定不同状态下线粒体呼吸功能.结果 Apelin-13 增强GK糖尿病大鼠BMSCs的ALP活性;促进钙结节矿化;与GK组比较,Apelin-13 有效提高两种状态下线粒体呼吸功能:复合物Ⅰ态 3 呼吸[(16.1±1.8)vs.(5.6±0.9)]pmol/(s×ml),复合物Ⅰ+复合物Ⅱ态 3 呼吸[(71.1±8.6)vs.(49.0±4.8)]pmol/(s×ml).结论 Apelin-13 可促进GK大鼠BMSCs骨向分化,并提高其线粒体呼吸功能.
Abstract
Objective To investigate the effect of Apelin-13 on osteoblast differentiation and mitochondrial respiration function of BMSCs derived from GK diabetic rats.Methods The BMSCs of GK diabetic rats were extracted.After cultured in osteogenic differentiation medium,the activity of alkaline phosphatase(ALP)was detected.Von Kossa staining was used to evaluate calcium nodules formation,and the mitochondrial respiratory function under different states was determined by O2K mitochondrial respiration apparatus.Results Apelin-13 enhanced the ALP activity of BMSCs in GK diabetic rats,and promoted calcium nodules mineralization.Compared with the GK group,Ape-lin-13 effectively improved mitochondrial respiratory function in two states:complex I state 3 respiration(16.1±1.8)vs.(5.6±0.9)]pmol/(s×ml)and complex Ⅰ+complex Ⅱ state 3 respiration[(71.1±8.6)vs.(49.0±4.8)]pmol/(s×ml).Conclusions Apelin-13 can promote osteoblast differentiation and enhance mitochondrial respiration function of BMSCs derived from GK diabetic rats.
关键词
爱帕琳肽13/骨髓间充质干细胞/线粒体呼吸Key words
Apelin-13/BMSCs/mitochondrial respiration引用本文复制引用
基金项目
北京市医管局青年项目(QML20230809)
出版年
2024
国家科技期刊平台
NSTL
万方数据