Effect of Apelin-13 on osteoblast differentiation and mitochondrial respiration function of BMSCs derived from GK diabetic rats
Objective To investigate the effect of Apelin-13 on osteoblast differentiation and mitochondrial respiration function of BMSCs derived from GK diabetic rats.Methods The BMSCs of GK diabetic rats were extracted.After cultured in osteogenic differentiation medium,the activity of alkaline phosphatase(ALP)was detected.Von Kossa staining was used to evaluate calcium nodules formation,and the mitochondrial respiratory function under different states was determined by O2K mitochondrial respiration apparatus.Results Apelin-13 enhanced the ALP activity of BMSCs in GK diabetic rats,and promoted calcium nodules mineralization.Compared with the GK group,Ape-lin-13 effectively improved mitochondrial respiratory function in two states:complex I state 3 respiration(16.1±1.8)vs.(5.6±0.9)]pmol/(s×ml)and complex Ⅰ+complex Ⅱ state 3 respiration[(71.1±8.6)vs.(49.0±4.8)]pmol/(s×ml).Conclusions Apelin-13 can promote osteoblast differentiation and enhance mitochondrial respiration function of BMSCs derived from GK diabetic rats.
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