Expression and clinical value of MAGE-A3 in cervical cancer tissues and PBMC
Objective To investigate the expression and clinical value of melanoma-associated antigen-A3(MAGE-A3)in cervical cancer tissues and peripheral blood mononuclear cells(PBMC).Methods A total of 96 patients with cervical conectomy or extensive hysterectomy due to cervical lesions from Baoding Maternal and Child Health Hospital and the Affiliated Hospital of Hebei U-niversity from May 2020 to May 2022 were selected by convenient sampling,and divided into cervical cancer group(56 cases)and cervical squamous intraepithelial lesion(SIL)group(40 cases)according to postoperative pathological results.Immunohistochemical method was used to detect the expression of MAGE-A3 protein in the pathological tissues.Real-time fluorescent quantitative PCR meth-od was used to detect the expression level of MAGE-A3mRNA in cervical lesion tissues and PBMC,and to analyze the correlation be-tween the expression of MAGE-A3 mRNA in cancer tissues and PBMC of cervical cancer patients and serum tumor markers,as well as the relationship between the expression of MAGE-A3 protein in cancer tissues and clinicopathological characteristics.To explore the di-agnostic value of MAGE-A3 mRNA expression in cancer tissue and PBMC for cervical cancer.Results The positive rate of MAGE-A3 protein expression in cervical cancer tissue was correlated with FIGO stage,tumor diameter,differentiation degree,lymph node metas-tasis and infection with high-risk HPV(P<0.05),but not with age or pathological type(P>0.05).The mRNA expression levels of MAGE-A3 in cancer tissue and PBMC in cervical cancer group were(1.33±0.46)and(1.70±0.49),respectively,which were sig-nificantly higher than those in SIL group(0.59±0.20)and PBMC group(0.92±0.33)(P<0.05).Serum levels of SCC-Ag[(3.87±1.69)ng/ml]and CA-125[(48.62±15.10)U/ml]were significantly higher than those of SIL group[(0.70±0.32)ng/ml,(25.36±8.33)U/ml](P<0.05).The expression positive rate of MAGE-A3 protein in cervical cancer group(66.07%)was significantly higher than that in SIL group(30.00%)(P<0.05).The mRNA ex-pression levels of MAGE-A3 in cancer tissue and PBMC of patients with cervical cancer were positively correlated with serum SCC-Ag and CA-125(P<0.05).Conclusions The expression of MAGE-A3 in cancer tissue and PBMC of patients with cervical cancer is up-regulated,and it is related to the level of serum tumor markers and dis-ease progression of patients.MAGE-A3 is expected to be an important marker for the diagnosis of early cervical cancer.
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