Effects of neuregulin 4 on apoptosis of aortic endothelial cells in type 2 diabetic mice
Objective To investigate the effects of neuregulin 4(Nrg4)on the apoptosis of endothelial cells in type 2 diabetic mice.Methods C57 mice were randomly divided into Vehicle group,diabetes mellites(DM)group and Nrg4 administration group(NRG4).Nrg4 group was intraperitoneally(i.p.)injected with Nrg4 recombinant protein(100 μg/kg,3 times/week)and the same volume of saline was i.p.injected in the other two groups for 8 weeks.After the experiment,TUNEL staining was used to evaluate ap-optotic rate of endothelial cells,and Western blot was used to investigate the protein levels of apoptosis-associated proteins.Results Compared with Vehicle group,DM significantly promoted cell apoptosis[(33.0±5.4)%vs.(20.5±3.0)%;P<0.05],in-creased Bax and cleaved-caspase 3 expression,and decreased Bcl-2 expression(P<0.05),while Nrg4 administration obviously de-creased endothelial apoptosis[(19.2±3.8)%vs.(33.0±5.4)%;P<0.05],decreased Bax and cleaved-caspase 3 expression,and increased Bcl-2 expression(P<0.05).Conclusions Nrg4 administration can alleviate the apoptosis of aortic endothelial cells in type 2 diabetic mice.
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