Determination of three β-blocker residues in meat by isotope labeling-ultra performance liquid chromatography-tandem mass spectrometry
Objective:To establish a MCX column purification method for the determination of three β-receptor blockers in meat products by ultra performance liquid chromatography tandem mass spectrometry.Method:After extraction by acid hydrolysis,pork samples were purified by Oasis MCX purification column.The mobile phases were eluted by gradient elution of methanol and 0.1％formic acid water,separated by ThermocHypersil Gold C18 column,multiple reaction monitoring(MRM)with ESI source positive ion mode,and quantified by internal standard method.Result:The linear range of the three β-receptor blockers was 0.5～18 μg/L,the correlation coefficient(r)was＞0.999,the LOD was 0.01～0.06 μg/kg,and the LOQ was 0.03～0.18μg/kg.At three spiked levels(0.4、1.6、3.2 μg/kg),the recoveries were 82.57％～103.22％,RSD were 3.10％～11.05％.Conclusion:This method is accurate and reliable,and can be used for qualitative and quantitative determination of β-receptor blockers in meat products.
NETL
NSTL
万方数据
维普
