DEHP promotes the malignant biological behavior of cervical cancer cells through the JAK-mediated activation of the Akt pathway
Objective To study the relationship between di-2-ethylexgl phthalate(DEHP)and the malignant biological behavior of cervical cancer cells.Methods The common pathological types of cervical malignant tumors human cervical adenocarcinoma cells Hela and squamous carcinoma cells Siha were exposed to different concentrations of DEHP.CCK-8 and clone formation assay were performed for cell viability.Hoechst 33258 staining were used to indicate apoptosis.Scratches and transwell experiments were used to show migration and invasion ability.Meanwhile,the expression and phosphorylation levels of the relevant proteins were detected by Western blot.The mRNA expression level of the JAK2(Janus 2 kinase)was explored by RT-quantitative PCR.Finally,the recovery of cells was observed by co-treatment of DEHP with the JAK2 inhibitor AG 490.Results After exposure to 102nmol/L or 103 nmol/L DEHP,cell viability,migration and invasion were significantly increased,and apoptosis was inhibited.The phosphorylation level of Akt was increased and the upstream JAK2 was upregulated.However,the JAK2 inhibitor alleviated DEHP-induced malignant biological behavior and the phosphorylation of Akt.Conclusion DEHP enhances the phosphorylation of Akt protein by inducing the expression of JAK2 and promotes the malignant biological behavior of cervical cancer cells.
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