Protective effect of extracts of Phyllanthus emblica L.on damage of PC 12 cells induced by high glucose via miR-34a
Objective To study the protective effect of extracts of Phyllanthus emblica L.on damage of PC 12 cells induced by high glucose and its related mechanisms.Methods PC 12 cells were damaged by high glucose induction,and then treated with extracts of Phyllanthus emblica L.or miR-34a was overexpressed by cell transfection.Methyl thiazolyl tetrazolium assay(MTT)was used to detect cell survival rate.Flow cytometry was used to detect cell apoptosis rate.Western blot was used to detect the protein expression levels of B-lymphocytoma-2-associated X protein(Bax)and B-lymphocytoma-2(Bcl-2).Fluorescence method was used to detect reactive oxygen species(ROS)level.Relevant kits were used to detect the levels of superoxide dismutase(SOD),glutathione peroxidase(GSH-Px),interleukin-6(IL-6)and interleukin-1β(IL-1β).Real-time quantitative PCR(qRT-PCR)was used to detect the expression levels of miR-34a.Results The survival rate,SOD and GSH-Px levels of PC 12 cells induced by high glucose were significantly increased by extracts of Phyllanthus emblica L.(LSD-t=-9.62,-9.64,-12.04,respectively,P＜0.05),while the apoptosis rate,Bax/Bcl-2 ratio,ROS,IL-6,IL-1β and miR-34a levels were significantly decreased(LSD-t=24.64,25.63,20.63,11.21,13.47,13.88,respectively,P＜0.05).Overexpression of miR-34a significantly reduced the survival rate,SOD and GSH-Px levels of PC 12 cells induced by high glucose(LSD-t=5.95,6.87,7.21,respectively,P＜0.05).While significantly increased the apoptosis rate,Bax/Bcl-2,ROS,IL-6 and IL-1βlevels(LSD-t=-9.64,-12.47,-6.69,-8.21,-8.99,respectively,P＜0.05).The extracts of Phyllanthus emblica L.could reverse the effect of miR-34a overexpression on high glucose-induced PC 12 cells.Conclusion The extracts of Phyllanthus emblica L.showed protective effect on damage of PC 12 cells induced by high glucose,and the mechanism may be related to the inhibition of miR-34a expression by extracts of Phyllanthus emblica L.and the reduction of oxidative stress and inflammation.
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