Effects of sub-acute exposure to cerium oxide nanoparticles on learning and memory ability in mice
Objective To investigate the effects of sub-acute exposure to cerium oxide nanoparticles on cognitive impairment in mice.Methods Morris water maze test were performed after intranasal instillation 30 days.The hippocampus was removed for Nissl staining and the contents of superoxidedismutase(SOD),malondialdehyde(MDA),γ-aminobutyric acid(GABA)and glutamate(Glu)in hippocampus were determined,the expression of NF-κB and NLRP3 by Western blot.Results Compared with the control group,the middle and high-dose groups had learning impairment in behavioral tests,and the middle and high dose groups had a significant reduction in the number of Nissl staining positive cells(F=9.246,P＜0.05).The activity of SOD in the high-dose group decreased,and the difference was statistically significant(F=3.969,P＜0.05).The content of MDA in the middle and high-dose groups increased,and the difference was statistically significant(F=9.368,P＜0.05).The content of GABA in each dose group decreased,and the differences were statistically significant(F=59.81,P＜0.05).The content of Glu in the middle and high dose groups increased,and the differences were statistically significant(F=10.64,P＜0.05).The expression of NF-κB and NLRP3 in the high dose group were significantly increased(F=10.97,F=7.443,P＜0.05).Conclusion Under the conditions of this test,sub-acute exposure to cerium oxide nanoparticles can affect the spatial learning ability of mice,damage hippocampal neurons,affect their antioxidant capacity and induce inflammatory response.
Cerium Oxide NanoparticlesLearning and memory abilitySub-acute Exposure
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