Neodymium content and distribution in ICR mice of a 28-day oral toxicity test for neodymium nitrate
Objective To determine the content of neodymium in whole blood and organs of ICR mice after 28 days of oral administration using Inductively Coupled Plasma Mass Spectrometry(ICP-MS).Methods Whole blood and organ samples were digested by nitric acid and microwave,and the content of neodymium was measured by ICP-MS with external standard method for quantification,to evaluate the content of rare earth element neodymium in the whole blood and organs of mice.Results The detection limit of neodymium element was 0.0018 μg/L,the recovery rate was between 97.2％and 111.0％,the relative standard deviation was between 1.63％and 7.93％,and the linear correlation coefficient was 0.9993.All indicators of this method met the relevant detection requirements.After continuous administration of neodymium nitrate for 28 days,the contents of neodymium in the heart,liver,and kidney of female mice began to rise significantly from a dosage of 12 mg/kg body weight(bw)(F values were 2289.00,37.09,and 903.50,respectively,P＜0.05),and in male mice from 7 mg/kg·bw(F values were 895.40,38.51,and 465.30,respectively,P＜0.05);the contents of neodymium in the lung and spleen of female mice began to rise significantly from a dosage of 24 mg/kg·bw(F values were 3702.00 and 94.66,respectively,P＜0.05),and in male mice from 14 mg/kg·bw(F values were 4988.00 and 104.00,respectively,P＜0.05);the content of neodymium in the brain of male mice began to rise significantly from a dosage of 27 mg/kg·bw(F value was 3.48,P＜0.05);the content of neodymium in the testes of male mice began to rise significantly from a dosage of 14 mg/kg·bw(F value was 8.28,P＜0.05).There was a statistically significant correlation between the contents of neodymium in the blood,brain,heart,lung,liver,spleen,kidney,and the administered dose(correlation coefficients r for female mice were 0.85,0.94,0.81,0.97,0.97,0.99,0.93,respectively,and for male mice were 0.95,0.97,0.81,0.91,0.96,0.97,0.95,and 0.98(for testicular neodymium content),respectively,P＜0.05).Conclusion The established detection method is accurate and sensitive.Neodymium nitrate administered orally can be distributed throughout the body via the bloodstream after intake in mice,with the highest distribution in the heart,liver,and kidney,followed by the lung,spleen,and testes,and lower content in the brain.There is a significant correlation between the content of neodymium in different organs and the administered dose.
Microwave digestionInductively coupled plasma mass spectrometryRare earth element neodymium
万方数据
维普
