摘要
目的 建立塑胶面层材料致大鼠肺损伤的动物模型,为后期毒性机制研究提供基础.方法 将检疫合格的SD大鼠按体重随机分为暴露组和对照组,每组20只,雌雄各半.采用吸入暴露方式进行染毒,每天吸入2 h,连续染毒90d.实验结束后,对大鼠进行剖检,测量血常规、凝血和血生化相应指标,称量脏器重量、计算脏体比,对脏器进行组织病理学检查.结果 染毒期间暴露组雄性大鼠的体重以及体重增量较对照组降低(t=2.436,P＜0.05).染毒结束后,与对照组比较,暴露组雌、雄大鼠肺重量、肺体比和脑体比均上升(t=-3.277,P＜0.01;t=-2.865,P＜0.05).脏器组织病理学检查结果显示,与对照组比较,暴露组雌、雄大鼠出现病理改变(间质性肺炎、肺出血和肺水肿)的动物数量明显增多(x2=5.000,P＜0.05).结论 在本实验条件下,根据脏器重量、脏体比以及肺病理改变判定塑胶面层材料致大鼠肺损伤模型成立.
Abstract
Objective A lung injury rat model induced by volatile organic compounds(VOC)released by plastic surface material was aim to establish to lay a foundation for future research of toxicity mechanism.Methods A total of 40 Sprague Dawley rats qualified for quarantine were randomized into 2 groups(20 rats for each group)by sex and weight.Rats were exposed to VOC released by plastic surface material,2 h/day for 90 days.Another group served as control.After exposure,serums were harvested for routine blood test,coagulation and blood biochemical indexes.Changes of visceral organs were studyed by calculating viscera-body ratio and histopathology examination.Results The body weight and weight gain of male rats in exposure group were lower than those in control group during exposure(t=2.436,P＜0.05).At the end of the experiment,the lung weight,lung-body ratio and brain-body ratio of both sexs in exposure group were increased compared with the control group(t=-3.277,P＜0.01;t=-2.865,P＜0.05).The result of organ histopathology showed that the number of histological abnormalities of both sexs with interstitial pneumonia,pulmonary hemorrhage and pulmonary edema in exposure group was significantly increased compared with the control(X2=5.000,P＜0.05).Conclusion Under the experimental conditions,the model of lung injury was established for the increase of organ weight and viscera-body ratio and pathological lung changes.
基金项目
广东省自然科学基金面上项目(2021A1515012494)
广东省市场监督管理局科技项目(2021CZ04)
广东省市场监督管理局科技项目(2020CZ03)
维普
万方数据