Response of Nostoc flagelliforme cell to Cu2+ , Cr2+ and Pb2 + stress
[Objective] This study aimed to investigate the effects of Cu2+,Cr2+ and Pb2+ stress on Nostocflagelliforme cell.[Methods] The response of Nostoc flagelliforme cell was analyzed under the stress.The modified BG11 culture medium containing different heavy metal ions of 0,0.1,1.0,10,100 mg/L was used to cultivate Nostocflagelliforme cell at 25℃ and light intensity of 80 μmol/(m· s).Electrolyte leakage,the activities of superoxide dismutase,the content of malondialdehyde,proline,soluble protein and trehalose were analyzed.[Results] Under 1-100 mg/L Cu2+,Cr2+ and Pb2+ stress,electrolyte leakage and malondialdehyde contents in Nostocflagelliforme cell were higher than those in the control group during heavy metal ions stress.Meanwhile,superoxide dismutase activity increased slightly under 10 mg/L,but was lower afterwards.The contents of proline,soluble protein and trehalose increased under 10mg/L heavy metal ions stress,while declined under extreme heavy metal ions stress (100 mg/L).[Conclusion] Nostocflagelliforme cell has resistance to low heavy metal ions stress,but is damaged badly under extreme heavy metal ions stress.
NETL
NSTL
万方数据
