首页|美洲大蠊(Periplaneta americana)肠道微生物多样性分析

美洲大蠊(Periplaneta americana)肠道微生物多样性分析

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[目的]分析美洲大蠊(Periplaneta americana)肠道微生物群落的组成.[方法]以美洲大蠊肠道微生物基因组为模板,Bact-27F和Univ-1492R为引物,PCR扩增16S rRNA基因,连接pGEM-T载体,构建肠道微生物16S rRNA基因文库,并对肠道微生物的组成及多样性进行分析.[结果]美洲大蠊肠道微生物主要包括变形杆菌门(Proteobacteria,66.4％),拟杆菌门(Bacteroidetes,17.8％),厚壁菌门(Firmicutes,14.5％),梭杆菌门(Fusobacteria,0.6％),以及未分类微生物(unclassified bacteria,0.6％).系统发育分析显示,15％的美洲大蠊肠道微生物16S rRNA基因序列与亲缘关系较近的杂食蟑螂肠道微生物的16S rRNA基因序列聚于一支;59％的美洲大蠊肠道微生物16S rRNA基因序列与不同食性动物肠道微生物的16S rRNA基因序列聚于一支.另一方面,18％的美洲大蠊肠道微生物16S rRNA基因序列与潜在的微生物致病菌一致性高于99％,说明美洲大蠊是一类潜在的致病菌携带者.[结论]美洲大蠊肠道微生物群落组成多样,宿主系统进化以及杂食性生活方式对其肠道微生物的组成有较大影响.
Phylogenetic analysis of bacterial community in the gut of American cockroach (Periplaneta americana)
[Objective] The present study was to fully evaluate the intestinal bacterial community of Periplaneta americana,an important model to study insects.[Methods] We investigated the bacterial community of P.americanagut by culture-independent methods,involving constructing the 16S rRNA gene library and microbial diversity analysis.[Results] The phylotypes were affiliated with Proteobacteria (66.4 ％),Bacteroidetes (17.8 ％),Firmicutes (14.5 ％),Fusobacteria (0.6％) and unclassified bacteria (0.6％).Phylogenetic analysis shows that 15％ of the sequences clustered with that from a closely related omnivorous cockroach;and 59％ clustered with that from more distantly related animals,including omnivorous,herbivorous,and carnivorous animals,which differ greatly in feeding habits.Moreover,18％ of the clones showed high sequence identity with potential pathogens closely related to human diseases,which also reinforces the concept of the cockroach as a carrier of pathogens.[Conclusion] Due to their habits of feeding on a variety of foodstuffs,omnivorous cockroaches harbor a large and diverse microbial community in the gut.The host phylogeny and dietary habits might be critical for the intestinal bacterial community composition of cockroaches.

American cockroach16S rRNA genemicrobial community

房伟、方泽民、刘智苗、袁璟、张学成、彭惠、洪宇植、肖亚中

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安徽大学生命科学学院,合肥230601

安徽省微生物与生物催化工程技术研究中心,合肥230601

美洲大蠊 16S rRNA基因 微生物群落

高等学校博士学科点专项科研基金安徽省高校省级自然科学研究项目安徽大学引进人才及学术带头人引进项目安徽大学211工程创新团队计划项目;Supported by the Specialized Research Fund for the Doctoral Program of Higher Education of China (200934011100

20093401110006KJ2010A02632030066

2013

微生物学报
中国科学院微生物研究所 中国微生物学会

微生物学报

CSTPCDCSCD北大核心
影响因子：0.857
ISSN：0001-6209
年,卷(期)：2013.53(9)
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