The impact of compound probiotics supplementation on gut microbiota structure,short-chain fatty acids profile,and inflammatory cytokine levels in Chinese wrestlers
The impact of compound probiotics supplementation on gut microbiota structure,short-chain fatty acids profile,and inflammatory cytokine levels in Chinese wrestlers
[Objective]To observe the effects of supplementing compound probiotics on the structure of gut microbiota,content of short-chain fatty acids(SCFAs),and levels of inflammatory cytokines in Chinese wrestlers.[Methods]Eighteen non-sports undergraduates from Shandong Sport University were recruited as a control group,while 30 Chinese wrestlers served as the experimental group.Both groups received oral compound probiotics for 8 weeks.Venous blood and stool samples were collected before and after the intervention.Enzyme-linked immunosorbent assay(ELISA)was used to measure inflammatory cytokines levels in plasma.The structural characteristics of gut microbiota were analyzed using 16S rRNA gene sequencing of the V3-V4 region,and gas chromatography-mass spectrometry(GC-MS)was employed to determine SCFAs content in stool samples.[Results]Prior to the intervention,the experimental group exhibited lower levels of interleukin-1(IL-1),interleukin-6(IL-6),and C reactive protein(CRP)(P＜0.001,P＜0.01,P＜0.01),and higher levels of interleukin-10(IL-10)compared to the control group(P＜0.001).After 8 weeks of probiotic supplementation,plasma levels of IL-6 and CRP in the Chinese wrestlers further declined(P＜0.01,P＜0.05).Before the intervention,the abundance of Bifidobacterium adolescentis was higher in Chinese wrestlers than that in the control group(P＜0.01).Following 8 weeks of supplementation,Chinese wrestlers showed an increased abundance of Collinsella(P＜0.01),a decreased abundance of Faecalibacterium(P＜0.01),and reduced a-diversity in gut microbiota(P＜0.01).Prior to the intervention,there was no significant difference in SCFAs content between the two groups.After 8 weeks of intervention with compound probiotics,the content of acetic acid and butyric acid in the stool samples increased in both the control group(P＜0.01,P＜0.05)and the experimental group(P＜0.05).The correlation analysis results indicated a positive correlation between B.adolescentis abundance and plasma IL-10 level(r=0.233,P=0.037)and negative correlations of B.adolescentis and C.aerofaciens abundance with plasma IL-6 level(r=-0.499,P=0.000;r=-0.366,P=0.001)in Chinese wrestlers.Additionally,there was a positive correlation between C.aerofaciens abundance and the butyric acid content in stool samples of Chinese wrestlers(r=0.243,P=0.032).[Conclusion]The 8 weeks intervention with compound probiotics effectively reduced pro-inflammatory cytokine levels and increased anti-inflammatory cytokine levels in plasma.Furthermore,it enhanced the abundance of butyric acid-producing bacteria in the gut microbiota,promoting the production of SCFAs,and improving anti-inflammatory capacity.
关键词
中国式摔跤/肠道菌群/短链脂肪酸/炎性因子
Key words
Chinese wrestling/gut microbiota/short-chain fatty acids/inflammatory cytokines
维普
万方数据